<p>Parabens, commonly used as preservatives in pharmaceuticals, personal care products, and food, pose environmental concerns due to their persistence and potential toxicity to aquatic organisms. In this study, the acute, chronic and multigenerational effects (following parental exposure) of butylparaben and hexylparaben were evaluated in <i>Moina macrocopa</i>. Acute toxicity tests revealed 48-hour LC₅₀ values of 9.50 (5.15–13.85) mg/L for butylparaben and 3.37 (0.16–6.58) mg/L for hexylparaben, indicating the higher toxicity of the latter. Chronic exposure of the parental (F0) genaration led to effects that persisted across unexposed offspring (F1–F3). Hexylparaben exhibited stronger toxic effects, with earlier mortality and greater inhibition of body development compared to butylparaben. Despite the persistent toxicity across generations, a slight recovery in survival and reproductive output was observed in F3, suggesting partial acclimation. These findings underscore the need for further investigation into the long-term ecological risks of parabens and their impact on aquatic populations.</p>