<p>The aim of this research was to determine the impact of preschool children’s temperament, their parents’ parenting attitudes, and their teachers’ self-regulation attitudes on children’s delay of gratification behavior. The study included 299 children aged 48–76 months, 149 girls and 150 boys, along with their parents and teachers. The average years of education for the mothers and fathers of the participants were 11.44 and 12.76, respectively. Children’s delay of gratification behavior was measured using the classic marshmallow test. Parents responded to attitude measures designed to reveal their own parenting attitudes and their children’s temperament characteristics. Teachers also responded to a scale designed to determine their self-regulation characteristics. When the children’s temperament characteristics, their parents’ parenting attitudes, and their teachers’ self-regulation characteristics were each independently associated with children’s delay of gratification behavior, no significant results were found. In independent associations, it was determined that only as the number of siblings increased, delay of gratification decreased. Beyond this, when the independent variables were modeled in relation to each other, it was also found that they were not significantly related to children’s delay of gratification behavior. Thus, this research suggests that maturation may play a role in the development of preschool children’s delay of gratification behavior rather than environmental factors. Therefore, the findings emphasize that contributions by parents and teachers to the development of delay of gratification behavior should start from an earlier age.</p>