Scholarlink

<p>Deepwater sculpin <i>Myoxocephalus thompsonii</i> is found in the Laurentian Great Lakes and deep, cold, oxygenated, oligotrophic lakes from the Gatineau region of Quebec to Great Slave and Great Bear lakes in northwestern Canada. Its distribution along the path of glacial retreat is patchy, although unknown populations may exist given the challenges of sampling deep lakes, especially in remote locations. Deepwater sculpin is of conservation concern, with two of four known populations in Quebec having been extirpated due to eutrophication, and the Great Lakes–Upper St. Lawrence population is listed as Special Concern under Canada’s <i>Species at Risk Act</i>. In this collaborative project between the Gatineau Fish and Game Club, SOI Foundation, Ottawa Riverkeeper, Canadian Museum of Nature, University of Manitoba, and GEN-FISH, we tested the ability of environmental DNA (eDNA) protocols to detect this species in 31 Mile Lake, Quebec, where it is known to occur. We then applied these methods to test for potential novel detections in Lac Pémichangan, which is adjacent but not connected to 31 Mile Lake. Deepwater sculpin eDNA was detected in water collected below the thermocline in August or September at 7/8 (2021), 5/5 (2022), and 2/6 (2023) sites in 31 Mile Lake. However, although detections in 2/162 quantitative PCR (qPCR) replicates were recorded in Lac Pémichangan in 2022, follow-up qPCRs suggested contamination. These eDNA protocols could be used to survey other candidate lakes for the presence of this elusive deepwater species and to monitor lakes where it is threatened by eutrophication, climate warming, or invasive species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Using environmental DNA (eDNA) and a multisector partnership to search for the elusive deepwater sculpin Myoxocephalus thompsonii

  • 期刊论文

  • 发表日期：

    2025年12月9日

查看全文
  • Margaret F. Docker,
  • Arfa A. Khan,
  • Jessie L. Ogden,
  • Roger D. Bull,
  • John Eydt,
  • Tamber Figurski,
  • Geoff Green

摘要

Deepwater sculpin Myoxocephalus thompsonii is found in the Laurentian Great Lakes and deep, cold, oxygenated, oligotrophic lakes from the Gatineau region of Quebec to Great Slave and Great Bear lakes in northwestern Canada. Its distribution along the path of glacial retreat is patchy, although unknown populations may exist given the challenges of sampling deep lakes, especially in remote locations. Deepwater sculpin is of conservation concern, with two of four known populations in Quebec having been extirpated due to eutrophication, and the Great Lakes–Upper St. Lawrence population is listed as Special Concern under Canada’s Species at Risk Act. In this collaborative project between the Gatineau Fish and Game Club, SOI Foundation, Ottawa Riverkeeper, Canadian Museum of Nature, University of Manitoba, and GEN-FISH, we tested the ability of environmental DNA (eDNA) protocols to detect this species in 31 Mile Lake, Quebec, where it is known to occur. We then applied these methods to test for potential novel detections in Lac Pémichangan, which is adjacent but not connected to 31 Mile Lake. Deepwater sculpin eDNA was detected in water collected below the thermocline in August or September at 7/8 (2021), 5/5 (2022), and 2/6 (2023) sites in 31 Mile Lake. However, although detections in 2/162 quantitative PCR (qPCR) replicates were recorded in Lac Pémichangan in 2022, follow-up qPCRs suggested contamination. These eDNA protocols could be used to survey other candidate lakes for the presence of this elusive deepwater species and to monitor lakes where it is threatened by eutrophication, climate warming, or invasive species.