Purpose <p>To investigate the effects of brief flickering light stimulation (FLS) on retinal electrophysiology and retinal blood flow (RBF) in normal C57BL6J mice.</p> Methods <p>RBF and full-field electroretinography (ffERG) were measured before and after a 60&#xa0;second FLS (12 Hz, 0.1 cd·s/m²) in a cohort of 8-12-weeks old C57BL6J mice (<i>n</i>=10) under anaesthetic and light-adapted conditions. A separate set of age-matched mice (<i>n</i>=9) underwent RBF and ffERG measurements before and after steady light stimulation (SLS) at 1 cd/m² under similar conditions. The changes in RBF (arterial and venous flow) as well as the amplitudes and implicit times of the a-wave, b-wave, oscillatory potentials (OPs), and photopic negative response (PhNR) were analyzed.</p> Results <p>FLS significantly increased both arterial (<i>p</i>=0.003) and venous (<i>p</i>=0.018) blood flow as well as b-wave amplitudes (<i>p</i>=0.017) compared to SLS, which did not have any significant changes in either RBF or ERG. However, no significant differences were found in other ffERG responses (amplitudes and implicit times of a-wave, OPs, and PhNR, as well as b-wave implicit time) between the two groups after light stimulation. An increase in b-wave amplitude was positively associated with an increase in both arterial (r=0.655, <i>p</i>=0.040) and venous blood flow (r=0.638, <i>p</i>=0.047) in the FLS group.</p> Conclusions <p>Our results suggest that transient FLS not only increases RBF but also enhances electro-retinal responses of the middle retinal layer, as shown by ffERG, thus demonstrating its substantial effects on both the vascular and neuronal components of retinal neurovascular coupling in mice.</p>