<p>Glioma is a malignant primary tumor of the brain. In recent years, numerous LncRNAs (Long non-coding RNA) have been demonstrated to be potential targets for glioma, and induction of pyroptosis is one of the important directions to inhibit the malignant process of cancer. The role of LncRNA PSMA-AS1, a novel lncRNA, on glioma remain unclear. The aim of this study was to investigate the effects and mechanisms of LncRNA PSMA-AS1 on glioma. PSMA1-AS1 and its potential targets were analyzed using bioinformatics tools and validated using a dual luciferase reporter gene system. Glioma cells were cultured to detect the expression levels of PSMA1-AS1 and its targets. The levels of PSMA1-AS1 and its targets were regulated by transfection and their effects on the viability and metastatic ability of glioma cells as well as on the level of pyroptosis were examined. PSMA1-AS1 expression was elevated in glioma, where miR-140-3p was a downstream target, and was decreased in glioma cells. Inhibition of PSMA1-AS1 promoted pyroptosis and inhibited glioma cell viability and metastatic ability. Overexpression of miR-140-3p had the same effect as inhibition of PSMA1-AS1, whereas inhibition of miR-140-3p reversed the effect of inhibition of PSMA1-AS1 on glioma cells. In addition, SRSF10 is a downstream binding target of miR-140-3p, and inhibition of SRSF10 also promoted pyroptosis and inhibited glioma cells proliferation and metastasis. In conclusion, our results confirmed that PSMA1-AS1 affects glioma cell proliferation and metastasis by regulating pyroptosis through miR-140-3p/SRSF10 axis, suggesting that LncRNA PSMA1-AS1 may be a potential target for glioma.</p>