Scholarlink

<p>Lung cancer is the most prevalent cancer and a major cause of cancer-related mortality worldwide. Long non-coding RNAs (lncRNAs) have been confirmed to be dysregulated in the progression of cancers. In this study, through RNA-next generation deep sequencing, we identified a new lncRNA (LINC01996), which was associated with prognosis of non-small cell lung cancer (NSCLC), downregulated in the NSCLC tissues and cell lines. We demonstrated that overexpressing LINC01996 inhibited NSCLC cell proliferation, EMT, and migration. Xenograft mouse models further validated the suppression role of LINC01996 in NSCLC progression and metastasis. By using differentially expressed genes in our RNA-next generation sequencing data, we discovered that LINC01996 was highly correlated with Cannabinoid receptor-interacting protein 1 (CNRIP1). Mechanistically, LINC01996 functions as a competitive endogenous RNA (ceRNA) by sponging miR-12115, thereby alleviating its repressive effect on CNRIP1 and leading to CNRIP1 upregulation. Overexpression of CNRIP1 ultimately inhibited Ras signaling pathway, leading to restrain of NSCLC proliferation and migration. In conclusion, LINC01996 can interact with miR-515-5p in the form of competitive endogenous RNA (ceRNA) to regulate CNRIP1 positively and thus affect the proliferation and metastasis of NSCLC cells. LINC01996/miR-12115/CNRIP1/Ras signaling axis plays a suppressive role in NSCLC progression and metastasis, and could serve as potential diagnostic markers and therapeutic targets for NSCLC.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

LINC01996 suppresses non-small cell lung cancer proliferation and metastasis by orchestrating the miR-12115/CNRIP1/Ras signaling axis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月17日

查看全文
  • Yi Zhang,
  • Xiaojing Li,
  • Shanshan Liu,
  • Qian Xue,
  • Peize Meng,
  • Xiaolu Ge,
  • Qingnan Zhao,
  • Haitao Ma

摘要

Lung cancer is the most prevalent cancer and a major cause of cancer-related mortality worldwide. Long non-coding RNAs (lncRNAs) have been confirmed to be dysregulated in the progression of cancers. In this study, through RNA-next generation deep sequencing, we identified a new lncRNA (LINC01996), which was associated with prognosis of non-small cell lung cancer (NSCLC), downregulated in the NSCLC tissues and cell lines. We demonstrated that overexpressing LINC01996 inhibited NSCLC cell proliferation, EMT, and migration. Xenograft mouse models further validated the suppression role of LINC01996 in NSCLC progression and metastasis. By using differentially expressed genes in our RNA-next generation sequencing data, we discovered that LINC01996 was highly correlated with Cannabinoid receptor-interacting protein 1 (CNRIP1). Mechanistically, LINC01996 functions as a competitive endogenous RNA (ceRNA) by sponging miR-12115, thereby alleviating its repressive effect on CNRIP1 and leading to CNRIP1 upregulation. Overexpression of CNRIP1 ultimately inhibited Ras signaling pathway, leading to restrain of NSCLC proliferation and migration. In conclusion, LINC01996 can interact with miR-515-5p in the form of competitive endogenous RNA (ceRNA) to regulate CNRIP1 positively and thus affect the proliferation and metastasis of NSCLC cells. LINC01996/miR-12115/CNRIP1/Ras signaling axis plays a suppressive role in NSCLC progression and metastasis, and could serve as potential diagnostic markers and therapeutic targets for NSCLC.