Scholarlink

<p>Repetitive DNA sequences, particularly satellite DNA (satDNA), are key components of eukaryotic genomes with important structural and potential functional roles. In this study, we used long-read assemblies and short-read sequencing to perform de novo identification of the most abundant satDNAs in the sea turtle <i>Caretta caretta</i>. We combined a bioinformatic approach and chromosome mapping to describe the distribution of the major tandem repetitive DNA sequences of this species. Additionally, we mapped major ribosomal DNA clusters, telomeric sequences and performed Chromomycin A3-DAPI-after G-banding (CDAG) staining to visualise AT-/GC-rich chromatin composition. We found that the C. <i>caretta</i> satellitome contains at least 18 satDNAs. Most of the satDNAs are localised in pericentromeric regions of chromosomes. We observed a differential, non-uniform distribution of repetitive DNA sequences between chromosomes: while most chromosomes carry blocks consisting of a single chromosome-specific satellite, some chromosomes contain complex heterochromatic blocks composed of multiple satellites. Comparative analysis showed inconsistencies between in situ and in silico satDNA mapping. These new data add to the knowledge about the diversity, distribution, and evolution of satDNA in the <i>C. caretta</i> genome.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Divergence and conservation of satellite DNA in the loggerhead sea turtle (Caretta caretta): a comparative cytogenomic analysis

  • 期刊论文

  • 发表日期：

    2026年2月23日

查看全文
  • Katerina V. Tishakova,
  • Anastasia A. Proskuryakova,
  • Dmitry Yu. Prokopov,
  • Sergey A. Marchenko,
  • Alessio Iannucci,
  • Claudio Ciofi,
  • Livia Tolve,
  • Paolo Casale,
  • Pasquale Salvemini,
  • Natalia A. Serdyukova,
  • Guzel I. Davletshina,
  • Vladimir A. Trifonov,
  • Svetlana A. Romanenko

摘要

Repetitive DNA sequences, particularly satellite DNA (satDNA), are key components of eukaryotic genomes with important structural and potential functional roles. In this study, we used long-read assemblies and short-read sequencing to perform de novo identification of the most abundant satDNAs in the sea turtle Caretta caretta. We combined a bioinformatic approach and chromosome mapping to describe the distribution of the major tandem repetitive DNA sequences of this species. Additionally, we mapped major ribosomal DNA clusters, telomeric sequences and performed Chromomycin A3-DAPI-after G-banding (CDAG) staining to visualise AT-/GC-rich chromatin composition. We found that the C. caretta satellitome contains at least 18 satDNAs. Most of the satDNAs are localised in pericentromeric regions of chromosomes. We observed a differential, non-uniform distribution of repetitive DNA sequences between chromosomes: while most chromosomes carry blocks consisting of a single chromosome-specific satellite, some chromosomes contain complex heterochromatic blocks composed of multiple satellites. Comparative analysis showed inconsistencies between in situ and in silico satDNA mapping. These new data add to the knowledge about the diversity, distribution, and evolution of satDNA in the C. caretta genome.