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<p>Cationic copolymer carboxymethyl cellulose-graft-poly(methacryloxyethyl trimethylammonium chloride) (CMC-<i>g</i>-PDMC) was synthesized via free radical polymerization and further modified with glutaraldehyde (GA) to immobilize horseradish peroxidase (HRP). DMC, a cationic monomer, was grafted onto CMC to enhance electrostatic adsorption, while GA provided covalent crosslinking sites for HRP. The GA-crosslinked copolymer (CMC-<i>g</i>-PDMC-GA) enabled efficient enzyme immobilization on glass surfaces for batch and continuous catalysis. After 10 cycles of batch catalysis, the immobilized HRP retained 98% of its initial activity. In continuous flow (2&#xa0;h), the activity remained stable, with a 1.6-fold higher efficiency on NaOH-treated glass compared to untreated surfaces. HRP alone or without GA crosslinking showed negligible immobilization.</p>

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Cationic modified carboxymethyl cellulose and its enzyme immobilization performance

  • 期刊论文

  • 发表日期：

    2025年6月17日

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  • Weimin Yan,
  • Chengmin Hou

摘要

Cationic copolymer carboxymethyl cellulose-graft-poly(methacryloxyethyl trimethylammonium chloride) (CMC-g-PDMC) was synthesized via free radical polymerization and further modified with glutaraldehyde (GA) to immobilize horseradish peroxidase (HRP). DMC, a cationic monomer, was grafted onto CMC to enhance electrostatic adsorption, while GA provided covalent crosslinking sites for HRP. The GA-crosslinked copolymer (CMC-g-PDMC-GA) enabled efficient enzyme immobilization on glass surfaces for batch and continuous catalysis. After 10 cycles of batch catalysis, the immobilized HRP retained 98% of its initial activity. In continuous flow (2 h), the activity remained stable, with a 1.6-fold higher efficiency on NaOH-treated glass compared to untreated surfaces. HRP alone or without GA crosslinking showed negligible immobilization.