<p>Transparency is an important characteristic for film products, yet it remains a formidable challenge in the realm of organic-inorganic hybrid and composite materials. Herein, we report the development of transparent TiO₂/cellulose hybrid films through an innovative in situ synthesis method, integrating TiO₂ formation during the regeneration process of cellulose. These hybrid films exhibit exceptional transparency, rivaling that of pure regenerated cellulose films, while simultaneously boasting a robust UV-shielding capacity. The successful integration of TiO₂ into the hybrid films was validated through XPS and Raman analysis, while XRD patterns revealed a decrease in the crystallinity of regenerated cellulose due to the introduction of TiO₂. To investigate the dye removal capabilities of these films, methylene blue was chosen as a model dye. Our experiments indicate that the incorporation of TiO₂ significantly enhances the dye absorption capacity and catalytic photodegradation activity of the regenerated cellulose film. Furthermore, antibacterial tests targeting <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> revealed that these hybrid films exhibit remarkable antibacterial properties, effectively inhibiting bacterial growth in both dark and UVC irradiation conditions. Collectively, these results underscore the promising applications of TiO₂/cellulose hybrid films in diverse fields, including dye wastewater treatment and active food packaging.</p> Graphical abstract <p></p>