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<p>The purpose of this study was to compare two methods for harvesting human dental pulp stem cells (hDPSCs) and investigate their impact on the DPSC viability and differentiation capacity. Healthy premolar teeth were collected from children aged 9 – 17&#xa0;years old undergoing orthodontic treatment with two or four premolar extractions. The included premolars were randomly allocated to the apical group (removal of the dental pulp through the apical foramen after tooth extraction) or the coronal group (isolating the pulp tissue through the crown prior to tooth extraction). A total of 148 healthy premolar teeth from 46 patients were extracted, including 74 pulp tissues for the apical group and 74 for the coronal group. Due to bacterial and fungal infections, 37 (18 apical and 19 coronal) DPSC lines were obtained. Flow cytometric analysis and MTT assays showed no statistical differences in cell viability between the two methods. Odontogenic differentiation, assessed through immunocytochemical staining and Alizarin Red staining, also showed no visual or statistical differences between the apical and coronal approaches. In conclusion, the biological characteristics of hDPSCs harvested through the crown were equal to apically prelevated hDPSCs.</p>

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Effect of the pulp harvesting method on the viability of human dental pulp stem cells and their odontogenic differentiation potential

  • 期刊论文

  • 发表日期：

    2025年7月21日

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  • Justine De Visscher,
  • Lore Vermeir,
  • Natasja Van den Vreken,
  • Liesbeth Temmerman,
  • Noëmi De Roo,
  • Jolanda van Hengel,
  • Guy De Pauw

摘要

The purpose of this study was to compare two methods for harvesting human dental pulp stem cells (hDPSCs) and investigate their impact on the DPSC viability and differentiation capacity. Healthy premolar teeth were collected from children aged 9 – 17 years old undergoing orthodontic treatment with two or four premolar extractions. The included premolars were randomly allocated to the apical group (removal of the dental pulp through the apical foramen after tooth extraction) or the coronal group (isolating the pulp tissue through the crown prior to tooth extraction). A total of 148 healthy premolar teeth from 46 patients were extracted, including 74 pulp tissues for the apical group and 74 for the coronal group. Due to bacterial and fungal infections, 37 (18 apical and 19 coronal) DPSC lines were obtained. Flow cytometric analysis and MTT assays showed no statistical differences in cell viability between the two methods. Odontogenic differentiation, assessed through immunocytochemical staining and Alizarin Red staining, also showed no visual or statistical differences between the apical and coronal approaches. In conclusion, the biological characteristics of hDPSCs harvested through the crown were equal to apically prelevated hDPSCs.