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<p>Chromosome-less minicells, derived from aberrant polar division events of bacterial cells, have emerged as promising nanocarriers for targeted cancer drug delivery due to their unique characteristics. A major challenge in their purification process lies in effectively isolating such spherical minicells (&lt; 1&#xa0;μm) from their rod-shaped parental cells (1–10&#xa0;μm). This study investigates the use of Deterministic Lateral Displacement (DLD) microfluidic systems for minicell purification, leveraging Two-Photon Lithography (TPL) for the rapid prototyping of high-resolution designs optimized for this purpose. Under laminar flow conditions, we investigated key DLD design parameters including symmetric and asymmetric post gaps, outlet widths, dual post arrays, fluidic-resistance-optimized design. To enhance separation efficiency, we developed a two-stage microfluidic separation system combining a spiral inertial chip and an optimized DLD chip in series. Utilizing high-resolution TPL for chip fabrication of an inertial chip with 12 spirals and an asymmetric DLD chip with a 2&#xa0;μm downstream post gap, we achieved a separation efficiency of 94%. This high efficiency achieved using microfluidics for the separation of cells differing in both shape and size, demonstrates the potential of advanced microfluidic systems in cell sorting.</p>

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Two-photon lithography-fabricated deterministic lateral displacement microfluidic system for efficient minicell purification in cancer therapy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月20日

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  • Sharaj Hegde Sharavu,
  • Sagar Bhagwat,
  • Sebastian Kluck,
  • Büsra Merve Kirpat Konak,
  • Barbara Di Ventura,
  • Pegah Pezeshkpour,
  • Bastian E. Rapp

摘要

Chromosome-less minicells, derived from aberrant polar division events of bacterial cells, have emerged as promising nanocarriers for targeted cancer drug delivery due to their unique characteristics. A major challenge in their purification process lies in effectively isolating such spherical minicells (< 1 μm) from their rod-shaped parental cells (1–10 μm). This study investigates the use of Deterministic Lateral Displacement (DLD) microfluidic systems for minicell purification, leveraging Two-Photon Lithography (TPL) for the rapid prototyping of high-resolution designs optimized for this purpose. Under laminar flow conditions, we investigated key DLD design parameters including symmetric and asymmetric post gaps, outlet widths, dual post arrays, fluidic-resistance-optimized design. To enhance separation efficiency, we developed a two-stage microfluidic separation system combining a spiral inertial chip and an optimized DLD chip in series. Utilizing high-resolution TPL for chip fabrication of an inertial chip with 12 spirals and an asymmetric DLD chip with a 2 μm downstream post gap, we achieved a separation efficiency of 94%. This high efficiency achieved using microfluidics for the separation of cells differing in both shape and size, demonstrates the potential of advanced microfluidic systems in cell sorting.