<p>Avocado (<i>Persea americana</i>) monoculture has triggered the loss of extensive forested areas in the Avocado Strip, the global core of Hass avocado production, but also a hotspot of conifer species richness, in central-western Mexico. This study assessed the potential habitat loss of conifer species due to the expansion of Hass avocado monoculture in the Avocado Strip. First, we modeled the distribution of Hass avocado and 26 conifer species (genera <i>Abies</i> and <i>Pinus</i>) through ecological niche modeling. Then, we delineated the areas covered by avocado orchards through photointerpretation of satellite imagery. Next, we intersected the layers of conifer distribution with suitable areas for Hass avocado cropping and avocado orchards and identified the sierras with the highest number of conifer species. Suitable areas for Hass avocado cropping extended over 21,703 km², of which 59% coincided with the distribution of conifer species. Moreover, 3,597 km² were covered by avocado orchards, of which 30% overlapped with the extent of at least one conifer species. Suitable areas for Hass avocado cropping overlapped with the distributions of 22 species, of which six overlapped by more than one-fifth of their extent (<i>Abies flinckii</i>, <i>Pinus douglasiana</i>, <i>P. maximinoi</i>, <i>P. lawsonii</i>, <i>P. jaliscana</i>, and <i>P. rzedowskii</i>). Furthermore, avocado orchards occupied up to 8% of the distribution range of all conifers. The two species with the most area occupied by avocado orchards were <i>A. flinckii</i> and <i>P. luzmariae</i>. Our findings highlight the expansion of avocado monoculture as a new threat to the habitat of 22 conifer species in Mexico.</p>