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<p>Strains of <i>S. cerevisiae</i> expressing GLP-1-GIP peptide precursors with an <i>N</i>-terminal extension demonstrate up to 29% higher molar productivity during continuous cultivation compared to non-extended counterparts. Additionally, inactivation of the <i>S. cerevisiae AMN1</i> gene, through deletion, effectively reduces broth viscosity by more than 45%, thereby enhancing oxygen transfer without adversely affecting cell metabolism or product titer. These results highlight the advantages of molecular modifications and genetic alterations for improving the production efficiency of GLP-1 receptor agonists, addressing critical challenges related to recombinant expression, viscosity and oxygen transfer in bioprocessing.</p>

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Evaluation of cultivation performance of S. cerevisiae strains expressing GLP-1-GIP peptide precursors

  • 期刊论文

  • 发表日期：

    2026年2月5日

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  • Ioannis Voulgaris,
  • Anders Nygaard Nielsen,
  • Tine Petersen,
  • Jakob Brandt,
  • Sanne Jensen

摘要

Strains of S. cerevisiae expressing GLP-1-GIP peptide precursors with an N-terminal extension demonstrate up to 29% higher molar productivity during continuous cultivation compared to non-extended counterparts. Additionally, inactivation of the S. cerevisiae AMN1 gene, through deletion, effectively reduces broth viscosity by more than 45%, thereby enhancing oxygen transfer without adversely affecting cell metabolism or product titer. These results highlight the advantages of molecular modifications and genetic alterations for improving the production efficiency of GLP-1 receptor agonists, addressing critical challenges related to recombinant expression, viscosity and oxygen transfer in bioprocessing.