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<p>To investigate the effects of fungal volatile organic compounds (FVOCs) on the mycelial growth of <i>Ganoderma lucidum</i>, and to elucidate the molecular mechanisms underlying the growth-promoting effect of 3-octanone. <i>G. lucidum</i> was cultivated with 1-octen-3-ol, 3-octanol and 3-octanone for 7&#xa0;days, after which colony diameter and mycelial dry weight were measured to assess their effects on mycelial growth. RNA-seq was used to investigate gene expression changes following 3-octanone exposure. While 1-octen-3-ol or 3-octanol inhibited mycelial growth in <i>G. lucidum</i>, 3-octanone promoted it. In total, 590 differentially expressed genes (DEGs), including 162 upregulated and 428 downregulated genes, were identified following 3-octanone exposure. Functional annotation revealed that among the DEGs, 23 genes were related to fungal cell wall biosynthesis and remodeling, whereas 21 genes were involved in plant-derived polysaccharide degradation. Furthermore, significant expression changes were observed in genes related to secondary metabolism. Our results indicate that <i>G. lucidum</i> can use 3-octanone as a signal to recognize other fungi, potentially facilitating the expansion of its own territory within wood in nature.</p>

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Gene expression profiling to elucidate the promotive effects of the volatile organic compound 3-octanone on the mycelial growth of Ganoderma lucidum

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月5日

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  • Shoko Horikawa,
  • Ryuka Iizuka,
  • Kiwamu Umezawa,
  • Rumi Konuma,
  • Makoto Yoshida

摘要

To investigate the effects of fungal volatile organic compounds (FVOCs) on the mycelial growth of Ganoderma lucidum, and to elucidate the molecular mechanisms underlying the growth-promoting effect of 3-octanone. G. lucidum was cultivated with 1-octen-3-ol, 3-octanol and 3-octanone for 7 days, after which colony diameter and mycelial dry weight were measured to assess their effects on mycelial growth. RNA-seq was used to investigate gene expression changes following 3-octanone exposure. While 1-octen-3-ol or 3-octanol inhibited mycelial growth in G. lucidum, 3-octanone promoted it. In total, 590 differentially expressed genes (DEGs), including 162 upregulated and 428 downregulated genes, were identified following 3-octanone exposure. Functional annotation revealed that among the DEGs, 23 genes were related to fungal cell wall biosynthesis and remodeling, whereas 21 genes were involved in plant-derived polysaccharide degradation. Furthermore, significant expression changes were observed in genes related to secondary metabolism. Our results indicate that G. lucidum can use 3-octanone as a signal to recognize other fungi, potentially facilitating the expansion of its own territory within wood in nature.