Objective
Development of a new phase function for measuring HbA1c, using a low-cost digital holographic microscopy to monitor the glucose level in diabetes.
Results
Here, we propose a new parameter, \({\text{\O }}_{cp}\) based on two different phases of RBCs, one, the absolute phase at the center, \({\text{\O }}_{c}\) and the other, the average phase at the periphery, \({\text{\O }}_{p}\) . The \({\text{\O }}_{cp}\) value varied almost linearly (R2 = 0.952) with HbA1c (%) in the blood. We used a low-cost digital holographic microscope (DHM) to record the hologram of RBCs, followed by digital reconstruction with the help of our newly introduced code. The critical value of \({\text{\O }}_{cp}\) at 3.5 was found to be equivalent to 6.5% HbA1c in high-performance liquid chromatography (HPLC), beyond which indicates hyperglycemia.
Conclusion
The method can be used for large-scale and low-cost estimation of HbA1c (%) with \({\text{\O }}_{cp}\) of ≥ 3.5.
