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Objective <p>Transglutaminase (TGase) is widely utilized in food, textile, and medical applications due to its superior capacity to facilitate protein cross-linking. The mechanisms driving high TGase production in <i>Streptomyces mobaraensis</i> require further exploration. “Microparticle-Enhanced Cultivation” (MPEC), has been shown to significantly influence the morphology of filamentous microorganisms, thereby impacting secondary metabolism.</p> Result <p>In the study, CaCO<sub>3</sub> and talc were found to increase the TGase activity of HVCP-Sm1 from 15 U/mL to 29.05 U/mL and 31 U/mL respectively. Moreover, CaCO<sub>3</sub> and talc were observed to reduce the diameters of hyphal particles and elongated hyphae while promoting pro-TGase activation. This research expands the understanding of the role of MPEC in the morphology of filamentous microorganisms and its effects on secondary metabolism.</p> Conclusion <p>The synergistic effects of group sensing, energy metabolism, and the optimization of mycelial morphology enhanced the secondary metabolism of HVCP-Sm1 and increased TGase enzymatic activity.</p>

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Improvement of transglutaminase production by controlling the morphology of Streptomyces mobaraensis HVCP-Sm1 with microparticle-enhanced cultivation

  • 期刊论文

  • 发表日期：

    2025年5月17日

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  • Ting-Ting Chang,
  • Lu-Yao Bian,
  • Chong Zhang

摘要

Objective

Transglutaminase (TGase) is widely utilized in food, textile, and medical applications due to its superior capacity to facilitate protein cross-linking. The mechanisms driving high TGase production in Streptomyces mobaraensis require further exploration. “Microparticle-Enhanced Cultivation” (MPEC), has been shown to significantly influence the morphology of filamentous microorganisms, thereby impacting secondary metabolism.

Result

In the study, CaCO3 and talc were found to increase the TGase activity of HVCP-Sm1 from 15 U/mL to 29.05 U/mL and 31 U/mL respectively. Moreover, CaCO3 and talc were observed to reduce the diameters of hyphal particles and elongated hyphae while promoting pro-TGase activation. This research expands the understanding of the role of MPEC in the morphology of filamentous microorganisms and its effects on secondary metabolism.

Conclusion

The synergistic effects of group sensing, energy metabolism, and the optimization of mycelial morphology enhanced the secondary metabolism of HVCP-Sm1 and increased TGase enzymatic activity.