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Objectives <p>This study aims to assess whether a fed-batch-based auto-induction method can enhance active hydrogenase production, encompassing cofactor formation and space–time yield.</p> Results <p>The recombinant <i>Escherichia coli</i> strain BQF8RH8, possessing plasmids for assembly and proper maturation of <i>Cupriavidus necator</i> regulatory hydrogenase (RH), was cultivated in the fed-batch like EnPresso B medium with an autoinduction lactose-based system. In contrast to classical IPTG induction previously performed, we obtained active RH demonstrating the feasibility of the process for active hydrogenase production.</p> Conclusion <p>Our results affirm the viability of the previously developed auto-induction strategy also for a functional hydrogenase with the complex maturation process. This significantly accelerates the process and enhances hydrogenase productivity.</p>

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Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月5日

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  • Francisco de la Fuente-Kratzborn,
  • Qin Fan,
  • Peter Neubauer,
  • Matthias Gimpel

摘要

Objectives

This study aims to assess whether a fed-batch-based auto-induction method can enhance active hydrogenase production, encompassing cofactor formation and space–time yield.

Results

The recombinant Escherichia coli strain BQF8RH8, possessing plasmids for assembly and proper maturation of Cupriavidus necator regulatory hydrogenase (RH), was cultivated in the fed-batch like EnPresso B medium with an autoinduction lactose-based system. In contrast to classical IPTG induction previously performed, we obtained active RH demonstrating the feasibility of the process for active hydrogenase production.

Conclusion

Our results affirm the viability of the previously developed auto-induction strategy also for a functional hydrogenase with the complex maturation process. This significantly accelerates the process and enhances hydrogenase productivity.