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<p>With the continuous advancement of technologies such as microbial cultivation, DNA sequencing, bioinformatics, and genetic engineering, in <i>vivo</i> mutagenesis methods based on perturbation factors are now widely utilized. We identified a RecJ enzyme (BaRecJ) with endonuclease and exonuclease activities from <i>Bacillus alcalophilus</i>, and established a broad-spectrum mutagenic method based on the endonuclease and exonuclease activities of BaRecJ. The BaRecJ mutagenesis method was applied to <i>S cerevisiae</i> to enhance its ethanol or acetic acid tolerance, resulting in mutant strains with improved fermentation performance. Genomic resequencing analysis summarizedgenes possibly associated with the tolerance of mutants. BaRecJ mutagenesis method not only holds immense potential in microbial mutagenesis breeding and adaptive evolution but also, when coupled with genomic resequencing, allows for the rapid identification of candidate genetic loci associated with specific traits.</p>

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Bacillus alcalophilus RecJ (BaRecJ) can drive the adaptive evolution of S. cerevisiae

  • 期刊论文

  • 发表日期：

    2025年7月22日

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  • Jixiang Shang,
  • Yanchao Zhang,
  • Zongjun Xu,
  • Shouqing Zhang,
  • Zhongtao Sun,
  • Minggang Zheng

摘要

With the continuous advancement of technologies such as microbial cultivation, DNA sequencing, bioinformatics, and genetic engineering, in vivo mutagenesis methods based on perturbation factors are now widely utilized. We identified a RecJ enzyme (BaRecJ) with endonuclease and exonuclease activities from Bacillus alcalophilus, and established a broad-spectrum mutagenic method based on the endonuclease and exonuclease activities of BaRecJ. The BaRecJ mutagenesis method was applied to S cerevisiae to enhance its ethanol or acetic acid tolerance, resulting in mutant strains with improved fermentation performance. Genomic resequencing analysis summarizedgenes possibly associated with the tolerance of mutants. BaRecJ mutagenesis method not only holds immense potential in microbial mutagenesis breeding and adaptive evolution but also, when coupled with genomic resequencing, allows for the rapid identification of candidate genetic loci associated with specific traits.