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<p>In Thailand, the median-striped bullfrog, <i>Kaloula mediolineata</i> is a commercially important amphibian because it is a natural food source that is easily accessible in rural areas. However, there is currently a dearth of knowledge, especially on the population diversity of this species. This study, therefore, aims to evaluate the genetic variation of <i>K. mediolineata</i> in Thailand using the partial cytochrome b (<i>Cyt-b</i>) and 16S ribosomal RNA (16S rRNA) sequences. A total of 118 samples from 20 localities (Provinces) were collected. <i>K. mediolineata</i> has been classified into 52 and 31&#xa0;haplotypes of <i>Cyt-b</i> haplotypes (Km1–Km52) and 16S rRNA haplotypes (Kr1–Kr31), respectively, which reflect high levels of genetic variation in this species. The 16S rRNA tree demonstrated a monophyletic group in our examined samples. Based on the <i>Cyt-b</i> sequence, the haplotype network and phylogenetic tree analyses showed that there are two separate genetic groups, which are named G1 and G2. These two divergent groups showed genetic differences with <i>p</i>-distance and <i>F</i><sub>ST</sub> values of 0.028 and 0.608 (<i>p</i>-value &lt; 0.001), respectively. Genetic group G1 is strictly found in certain localities in the north, central, and east regions, whereas G2 is most widespread in this study. Two specimens showed high genetic differences from the others, which indicated that other cryptic genetic groups may exist. Thus, a comprehensive investigation of the biology, ecology, as well as genetic variation of <i>K. mediolineata</i> could be further conducted.</p>

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Mitochondrial DNA Sequences Variation of the Median-Striped Bullfrog Kaloula mediolineata Smith, 1917 (Amphibia: Microhylidae) in Thailand

  • 期刊论文

  • 发表日期：

    2025年5月17日

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  • Maneesila Photiwatnangun,
  • Anchalee Aowphol,
  • Weerachai Saijuntha,
  • Komsorn Lauprasert

摘要

In Thailand, the median-striped bullfrog, Kaloula mediolineata is a commercially important amphibian because it is a natural food source that is easily accessible in rural areas. However, there is currently a dearth of knowledge, especially on the population diversity of this species. This study, therefore, aims to evaluate the genetic variation of K. mediolineata in Thailand using the partial cytochrome b (Cyt-b) and 16S ribosomal RNA (16S rRNA) sequences. A total of 118 samples from 20 localities (Provinces) were collected. K. mediolineata has been classified into 52 and 31 haplotypes of Cyt-b haplotypes (Km1–Km52) and 16S rRNA haplotypes (Kr1–Kr31), respectively, which reflect high levels of genetic variation in this species. The 16S rRNA tree demonstrated a monophyletic group in our examined samples. Based on the Cyt-b sequence, the haplotype network and phylogenetic tree analyses showed that there are two separate genetic groups, which are named G1 and G2. These two divergent groups showed genetic differences with p-distance and FST values of 0.028 and 0.608 (p-value < 0.001), respectively. Genetic group G1 is strictly found in certain localities in the north, central, and east regions, whereas G2 is most widespread in this study. Two specimens showed high genetic differences from the others, which indicated that other cryptic genetic groups may exist. Thus, a comprehensive investigation of the biology, ecology, as well as genetic variation of K. mediolineata could be further conducted.