Scholarlink

Abstract <p>Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases affecting the global pig industry. Host microRNAs directly target viral gene regions to exert their disease-fighting effects. PRRS virus (PRRSV) infection upregulates <i>miR-361-3p</i> expression; however, it is unclear whether it can exert inhibitory effects by directly targeting viral genes. Bioinformatic and experimental findings revealed that <i>miR-361-3p</i> inhibited PRRSV replication by directly targeting the PRRSV <i>ORF1b</i> and <i>ORF1a</i> loci. Intramuscular injection of pcDNA3.1-pri-<i>miR-361</i> verified the expression of <i>miR-361-3p</i> in mammals. In summary, <i>miR-361-3p</i> plays an important role in infection and may be a promising therapeutic target for PRRS, providing insights into possible drug therapies.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

ssc-miR-361-3p Suppresses Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Replication and Its In Vivo Expression in Mice

  • 期刊论文

  • 发表日期：

    2025年2月19日

查看全文
  • Qinchuan Zhang,
  • Manyi Zhang,
  • Xiao Qi,
  • Jinliang Sheng,
  • Yanming Sun,
  • Yanbing Zhang

摘要

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases affecting the global pig industry. Host microRNAs directly target viral gene regions to exert their disease-fighting effects. PRRS virus (PRRSV) infection upregulates miR-361-3p expression; however, it is unclear whether it can exert inhibitory effects by directly targeting viral genes. Bioinformatic and experimental findings revealed that miR-361-3p inhibited PRRSV replication by directly targeting the PRRSV ORF1b and ORF1a loci. Intramuscular injection of pcDNA3.1-pri-miR-361 verified the expression of miR-361-3p in mammals. In summary, miR-361-3p plays an important role in infection and may be a promising therapeutic target for PRRS, providing insights into possible drug therapies.

Graphical Abstract