Scholarlink

<p><i>Spodoptera exigua</i> (Hübner) (Lepidoptera: Noctuidae) is a destructive lepidopteran pest commonly managed using microbial biopesticides such as <i>Bacillus thuringiensis</i> (Bt), entomopathogenic fungi, and nucleopolyhedroviruses (NPVs). However, the relatively slow speed of action and variable efficacy of these agents can limit their broader application. Vip3Aa35, a vegetative insecticidal protein secreted by Bt strain M190, exhibits strong toxicity against lepidopteran larvae. This study evaluated the potential of Vip3Aa35 to enhance the efficacy of three microbial control agents, Xentari® (Bt-based), <i>Beauveria bassiana</i> BCC 48145, and <i>Spodoptera exigua</i> nucleopolyhedrovirus (SeNPV), under laboratory and greenhouse conditions. When combined with Xentari®, Vip3Aa35 increased overall insecticidal performance and reduced larval killing time. In combination with <i>B. bassiana</i> at sub-lethal concentrations (62.5–125&#xa0;ng&#xa0;cm<sup>–2</sup>), Vip3Aa35 markedly enhanced fungal virulence and accelerated larval mortality. Co-application with SeNPV substantially reduced the LC<sub>50</sub> and shortened LT<sub>50</sub> values, indicating faster viral infection and killing. Greenhouse experiments further demonstrated that Vip3Aa35, even at low application rates (17.78&#xa0;µg pot<sup>–1</sup>), improved plant protection when combined with <i>B. bassiana</i> or SeNPV, resulting in reduced leaf damage and more rapid larval death. Early visual symptoms, including fungal outgrowth and virus-induced larval cadavers, were observed sooner in combined treatments than in single-agent applications. Overall, these findings demonstrate that Vip3Aa35 can enhance the performance of fungal, bacterial, and viral biocontrol agents against <i>S. exigua</i>, supporting its potential use as a synergistic component in integrated pest management strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Vip3Aa35 enhances the efficacy of Bacillus thuringiensis, Beauveria bassiana, and SeNPV for Spodoptera exigua control: a synergistic approach for sustainable pest management

  • 期刊论文

  • 发表日期：

    2026年4月28日

查看全文
  • Mongkon Utamatho,
  • Sumarin Soonsnga,
  • Cheerapha Panyasiri,
  • Amporn Rungrod,
  • Samrit Kiewwong,
  • Boonhiang Promdonkoy

摘要

Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) is a destructive lepidopteran pest commonly managed using microbial biopesticides such as Bacillus thuringiensis (Bt), entomopathogenic fungi, and nucleopolyhedroviruses (NPVs). However, the relatively slow speed of action and variable efficacy of these agents can limit their broader application. Vip3Aa35, a vegetative insecticidal protein secreted by Bt strain M190, exhibits strong toxicity against lepidopteran larvae. This study evaluated the potential of Vip3Aa35 to enhance the efficacy of three microbial control agents, Xentari® (Bt-based), Beauveria bassiana BCC 48145, and Spodoptera exigua nucleopolyhedrovirus (SeNPV), under laboratory and greenhouse conditions. When combined with Xentari®, Vip3Aa35 increased overall insecticidal performance and reduced larval killing time. In combination with B. bassiana at sub-lethal concentrations (62.5–125 ng cm–2), Vip3Aa35 markedly enhanced fungal virulence and accelerated larval mortality. Co-application with SeNPV substantially reduced the LC50 and shortened LT50 values, indicating faster viral infection and killing. Greenhouse experiments further demonstrated that Vip3Aa35, even at low application rates (17.78 µg pot–1), improved plant protection when combined with B. bassiana or SeNPV, resulting in reduced leaf damage and more rapid larval death. Early visual symptoms, including fungal outgrowth and virus-induced larval cadavers, were observed sooner in combined treatments than in single-agent applications. Overall, these findings demonstrate that Vip3Aa35 can enhance the performance of fungal, bacterial, and viral biocontrol agents against S. exigua, supporting its potential use as a synergistic component in integrated pest management strategies.