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<p>Covert infections pose significant challenges to global insect production systems, compromising the reliability of biological research, mass rearing of biological control agents and edible insect farming. In this study, we characterized a Trichoplusia ni cypovirus 15 (TnCPV15)-like isolate infecting key Brazilian soybean pests through stressor-mediated activation in Noctuoidea hosts. Using electrophoretic profiling, electron microscopy, high-throughput sequencing, we identified a CPV isolate meeting ICTV species demarcation criteria for <i>Cypovirus trichoplusiae</i> based on: (1) conserved 10 dsRNA segment pattern, (2) 88–92% <i>polyhedrin</i> gene identity to TnCPV15, (3) phylogenetic clustering with reference strains, (4) terminal motif 5′-AUUAAA and 3′-GC in most of the segments. Among tested stressors (copper sulfate, sodium selenite, iron sulfate, and <i>Bacillus thuringiensis</i>), copper sulfate most effectively triggered CPV activation without excessive mortality (≤ 23.3% across species). Infection prevalence varied significantly among hosts: highest in <i>Anticarsia gemmatalis</i> Hübner (Lepidoptera: Erebidae), followed by <i>Rachiplusia nu</i> (Lepidoptera: Noctuidae) and <i>Chrysodeixis includens</i> (Lepidoptera: Noctuidae), while <i>Spodoptera frugiperda</i> (Lepidoptera: Noctuidae) showed low susceptibility. These findings demonstrate how stressor exposure can reveal covert infections in rearing systems while highlighting a CPV isolate with potential for selective pest management. The isolates’ lethality in key soybean pests but not in <i>S. frugiperda</i> suggests possible applications in integrated pest management programs.</p>

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Characterization of a cypovirus isolate in Noctuoidea pests: host range, and stressor interactions

  • 期刊论文

  • 发表日期：

    2026年1月8日

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  • Alini de Almeida,
  • Andrews Alexander Fredéric Monvosin Santos Fisch,
  • Luis Janssen,
  • Leonardo Assis da Silva,
  • Maria Cristina Neves de Oliveira,
  • Daniel Mendes Pereira Ardisson-Araújo,
  • Bergmann Morais Ribeiro,
  • Daniel R. Sosa-Gómez

摘要

Covert infections pose significant challenges to global insect production systems, compromising the reliability of biological research, mass rearing of biological control agents and edible insect farming. In this study, we characterized a Trichoplusia ni cypovirus 15 (TnCPV15)-like isolate infecting key Brazilian soybean pests through stressor-mediated activation in Noctuoidea hosts. Using electrophoretic profiling, electron microscopy, high-throughput sequencing, we identified a CPV isolate meeting ICTV species demarcation criteria for Cypovirus trichoplusiae based on: (1) conserved 10 dsRNA segment pattern, (2) 88–92% polyhedrin gene identity to TnCPV15, (3) phylogenetic clustering with reference strains, (4) terminal motif 5′-AUUAAA and 3′-GC in most of the segments. Among tested stressors (copper sulfate, sodium selenite, iron sulfate, and Bacillus thuringiensis), copper sulfate most effectively triggered CPV activation without excessive mortality (≤ 23.3% across species). Infection prevalence varied significantly among hosts: highest in Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae), followed by Rachiplusia nu (Lepidoptera: Noctuidae) and Chrysodeixis includens (Lepidoptera: Noctuidae), while Spodoptera frugiperda (Lepidoptera: Noctuidae) showed low susceptibility. These findings demonstrate how stressor exposure can reveal covert infections in rearing systems while highlighting a CPV isolate with potential for selective pest management. The isolates’ lethality in key soybean pests but not in S. frugiperda suggests possible applications in integrated pest management programs.