Scholarlink

<p>Microbial biopesticides play a crucial role in integrated disease management by offering sustainable alternatives to synthetic chemicals, thereby reducing their use. This study aimed to develop a broad-spectrum <i>Trichoderma</i> (Hypocreales: Hypocreaceae) biopesticide against multiple tea (<i>Camellia sinensis</i>) pathogens and to evaluate its effectiveness in field. A total of 149 isolates of <i>Trichoderma</i> spp. were isolated from tea soils across different locations in Sri Lanka. Tric 33, the most promising isolate, was identified as <i>T. asperellum</i> through morphology and molecular sequencing of the ITS and <i>tef-1α</i> regions. In vitro dual culture antagonistic assays showed that <i>T. asperellum</i> Tric 33 suppressed the growth of <i>Poria hypolateritia</i>, <i>Rosellinia arcuata</i>, <i>Phellinus noxius</i> (root pathogens), <i>Lasiodiplodia theobromae</i>, <i>Fusarium solani</i> species complex, <i>Marasmius crinis-equi</i> (stem pathogens), <i>Colletotrichum</i> sp. and <i>Pestalotiopsis</i> sp. (foliar pathogens) by 76–89%. Growth assessments indicated <i>T. asperellum</i> Tric 33 thrives at pH 4.5–5.5 and at temperatures between 21–28 °C. While dolomite and glyphosate did not negatively impact the growth of <i>T. asperellum</i> Tric 33, copper fungicide and hexaconazole inhibited its growth. Field assessments of a wettable powder formulation of <i>T. asperellum</i> Tric 33 (10<sup>8</sup>&#xa0;CFU g<sup>−1</sup>) confirmed its field efficacy against multiple pathogens, establishing it as a sustainable alternative to synthetic fungicides in integrated disease management. Incorporation of <i>T. asperellum</i> Tric 33 into integrated disease management strategies would promote biodiversity, preserve soil health and support climate-smart cultivation practices.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Trichoderma asperellum Tric 33 as a broad spectrum biopesticide against multiple diseases of tea (Camellia sinensis (L.) O. Kuntze)

  • 期刊论文

  • 发表日期：

    2025年12月15日

查看全文
  • R. D. S. M. Gamlath,
  • K. L. S. C. Gunawardana,
  • D. G. N. P. Karunajeewa,
  • N. Mahadevan,
  • P. D. Senanayake,
  • S. Rajapakse,
  • G. D. Sinniah

摘要

Microbial biopesticides play a crucial role in integrated disease management by offering sustainable alternatives to synthetic chemicals, thereby reducing their use. This study aimed to develop a broad-spectrum Trichoderma (Hypocreales: Hypocreaceae) biopesticide against multiple tea (Camellia sinensis) pathogens and to evaluate its effectiveness in field. A total of 149 isolates of Trichoderma spp. were isolated from tea soils across different locations in Sri Lanka. Tric 33, the most promising isolate, was identified as T. asperellum through morphology and molecular sequencing of the ITS and tef-1α regions. In vitro dual culture antagonistic assays showed that T. asperellum Tric 33 suppressed the growth of Poria hypolateritia, Rosellinia arcuata, Phellinus noxius (root pathogens), Lasiodiplodia theobromae, Fusarium solani species complex, Marasmius crinis-equi (stem pathogens), Colletotrichum sp. and Pestalotiopsis sp. (foliar pathogens) by 76–89%. Growth assessments indicated T. asperellum Tric 33 thrives at pH 4.5–5.5 and at temperatures between 21–28 °C. While dolomite and glyphosate did not negatively impact the growth of T. asperellum Tric 33, copper fungicide and hexaconazole inhibited its growth. Field assessments of a wettable powder formulation of T. asperellum Tric 33 (108 CFU g−1) confirmed its field efficacy against multiple pathogens, establishing it as a sustainable alternative to synthetic fungicides in integrated disease management. Incorporation of T. asperellum Tric 33 into integrated disease management strategies would promote biodiversity, preserve soil health and support climate-smart cultivation practices.