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<p>The global spread of multidrug-resistant pathogens underscores the urgent need for novel therapeutic strategies against bacterial infections. One promising approach is phage therapy, which utilizes lytic bacteriophages that exhibit high specificity for their bacterial hosts. In this study, we isolated a novel bacteriophage, vB_EcoA_PHZVL, belonging to the family <i>Autographiviridae</i>, which demonstrates potent lytic activity against a clinical strain of adherent-invasive <i>Escherichia coli</i> (AIEC) isolated from a patient with Crohn’s disease (CD). We further demonstrated that both plaque morphology and phage adsorption efficiency are influenced by the composition of the culture medium and the carbon source, likely due to alterations in LPS structure on the bacterial surface. These findings enhance our understanding of phage–host interactions and highlight the potential of vB_EcoA_PHZVL as a candidate for phage-based therapy in CD.</p>

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A Novel Bacteriophage Targeting Adherent-Invasive Escherichia coli: A Potential Therapeutic Agent for Crohn’s Disease

  • 期刊论文

  • 发表日期：

    2025年11月1日

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  • M. V. Mikhailycheva,
  • A. A. Semenov,
  • N. I. Borzenko,
  • A. S. Avshalumov,
  • D. R. Urazaeva,
  • M. A. Galyamina,
  • R. B. Gorodnichev,
  • O. V. Pobeguts,
  • A. Yu. Gorbachev

摘要

The global spread of multidrug-resistant pathogens underscores the urgent need for novel therapeutic strategies against bacterial infections. One promising approach is phage therapy, which utilizes lytic bacteriophages that exhibit high specificity for their bacterial hosts. In this study, we isolated a novel bacteriophage, vB_EcoA_PHZVL, belonging to the family Autographiviridae, which demonstrates potent lytic activity against a clinical strain of adherent-invasive Escherichia coli (AIEC) isolated from a patient with Crohn’s disease (CD). We further demonstrated that both plaque morphology and phage adsorption efficiency are influenced by the composition of the culture medium and the carbon source, likely due to alterations in LPS structure on the bacterial surface. These findings enhance our understanding of phage–host interactions and highlight the potential of vB_EcoA_PHZVL as a candidate for phage-based therapy in CD.