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<p>The growth-stimulating effect of platelet lysates obtained after exposure of platelet suspension to low-pulse laser radiation (LPLR) with λ = 470 nm (blue laser) and λ = 635 nm (red laser) was studied in a culture of human M-22 fibroblasts. LPLR-irradiated platelet suspension in plasma and plasma-free medium reduced the content of platelets with granules by 5-7% at λ = 470 nm and 25-27% at λ = 635 nm (<i>p</i> &lt; 0.05). In presence of platelet lysates, the number of fibroblasts significantly increased by 1.3-1.4 times after 3 days of cultivation. In experiments with platelets in plasma-free medium, the samples previously irradiated at λ = 470 nm produced a lower growth-stimulating effect, than samples irradiated at λ = 635 nm and non-irradiated samples; for platelets irradiated in autologous plasma, no prominent differences were observed. Lysates of LPLR-irradiated platelets did not impair structural integrity of human fibroblasts in culture.</p>

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Growth-Stimulating Effect of Platelet Lysates Obtained with Low-Pulse Laser Radiation in Human Fibroblast Culture

  • 期刊论文

  • 发表日期：

    2025年10月1日

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  • M. S. Makarov,
  • M. V. Storozheva,
  • N. V. Borovkova,
  • I. N. Ponomarev

摘要

The growth-stimulating effect of platelet lysates obtained after exposure of platelet suspension to low-pulse laser radiation (LPLR) with λ = 470 nm (blue laser) and λ = 635 nm (red laser) was studied in a culture of human M-22 fibroblasts. LPLR-irradiated platelet suspension in plasma and plasma-free medium reduced the content of platelets with granules by 5-7% at λ = 470 nm and 25-27% at λ = 635 nm (p < 0.05). In presence of platelet lysates, the number of fibroblasts significantly increased by 1.3-1.4 times after 3 days of cultivation. In experiments with platelets in plasma-free medium, the samples previously irradiated at λ = 470 nm produced a lower growth-stimulating effect, than samples irradiated at λ = 635 nm and non-irradiated samples; for platelets irradiated in autologous plasma, no prominent differences were observed. Lysates of LPLR-irradiated platelets did not impair structural integrity of human fibroblasts in culture.