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<p>The protective properties of argon-oxygen mixture were studied in the culture of M-22 human fibroblasts, piglet thymus cells, and rat brain cells. To induce damage to cell membranes, 200 μM H<sub>2</sub>O<sub>2</sub> and 500 μM acetic acid were used. The cells were exposed in the presence of a gas mixture containing 70% argon and 30% oxygen (ArgOx 70/30) at 37°C for 6 h. The ArgOx 70/30 mixture produced no toxic effect on the cells of all studied cultures. In culture of M-22 fibroblasts, acetic acid-induced cell death decreased by 1.5-1.9 times under the influence of ArgOx 70/30 and the cell membranes remained better preserved. In none cultures, ArgOx 70/30 mixture significantly protected cells from hydrogen peroxide-induced damage. Piglet thymus culture cells demonstrated very high resistance to high concentrations of hydrogen peroxide and acetic acid, while rat brain cells, on the contrast, had low resistance.</p>

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Effectiveness of Argon–Oxygen Mixture for Maintaining the Viability of Cultured Cells under the Influence of Membrane-Damaging Factors

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • N. V. Borovkova,
  • A. K. Shabanov,
  • M. S. Makarov,
  • M. V. Storozheva,
  • I. N. Ponomarev,
  • A. K. Evseev,
  • O. A. Grebenchikov,
  • R. A. Cherpakov,
  • S. S. Petrikov

摘要

The protective properties of argon-oxygen mixture were studied in the culture of M-22 human fibroblasts, piglet thymus cells, and rat brain cells. To induce damage to cell membranes, 200 μM H2O2 and 500 μM acetic acid were used. The cells were exposed in the presence of a gas mixture containing 70% argon and 30% oxygen (ArgOx 70/30) at 37°C for 6 h. The ArgOx 70/30 mixture produced no toxic effect on the cells of all studied cultures. In culture of M-22 fibroblasts, acetic acid-induced cell death decreased by 1.5-1.9 times under the influence of ArgOx 70/30 and the cell membranes remained better preserved. In none cultures, ArgOx 70/30 mixture significantly protected cells from hydrogen peroxide-induced damage. Piglet thymus culture cells demonstrated very high resistance to high concentrations of hydrogen peroxide and acetic acid, while rat brain cells, on the contrast, had low resistance.