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<p>The study examined implication of miRNA-19b carried by endothelial microparticles (EMPs) in phenotypic switching of vascular smooth muscle cells (VSMCs) and the mechanisms underlying this transformation. The functions of miRNA-19b were assessed by phenotypic switching, proliferation, and migration of VSMCs. The target genes of miR‐19b associated with proliferation and migration were revealed by analysis of TargetScan and miRanda databases and verified with luciferase assay. Experiments showed that EMPs could transfer miRNA-19b into VSMCs. Elevated content of miRNA-19b increased expression of contractile phenotypic markers SMA and SM22α and inhibited proliferation and migration of VSMCs. The direct target gene of miRNA-19b turned out to be the mitogen-activated protein kinase 6 (<i>MAPK6</i>). Thus, miRNA-19b in EMPs could inhibit phenotypic transformation of VSMCs from the contractile phenotype to synthetic one and reduce proliferation and migration by down-regulating <i>MAPK6</i> expression, which can potentially inhibit the development of atherosclerosis.</p>

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Effects of MicroRNA-19b Carried by Endothelial Microparticles on the Phenotypic Switching of Vascular Smooth Muscle Cells and the Working Mechanism

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Yuxia Cui,
  • Junxian Song,
  • Ting Ge,
  • Manyan Wu,
  • Chongyou Lee,
  • Hong Chen

摘要

The study examined implication of miRNA-19b carried by endothelial microparticles (EMPs) in phenotypic switching of vascular smooth muscle cells (VSMCs) and the mechanisms underlying this transformation. The functions of miRNA-19b were assessed by phenotypic switching, proliferation, and migration of VSMCs. The target genes of miR‐19b associated with proliferation and migration were revealed by analysis of TargetScan and miRanda databases and verified with luciferase assay. Experiments showed that EMPs could transfer miRNA-19b into VSMCs. Elevated content of miRNA-19b increased expression of contractile phenotypic markers SMA and SM22α and inhibited proliferation and migration of VSMCs. The direct target gene of miRNA-19b turned out to be the mitogen-activated protein kinase 6 (MAPK6). Thus, miRNA-19b in EMPs could inhibit phenotypic transformation of VSMCs from the contractile phenotype to synthetic one and reduce proliferation and migration by down-regulating MAPK6 expression, which can potentially inhibit the development of atherosclerosis.