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<p>The parameters of DNA-containing extracellular structures formed by immortalized fibroblasts and HCT116 adenocarcinoma cells during cultivation were studied. Fluorescence microscopy with SYBR Green dye was used to visualize DNA-containing extracellular structures. In contrast to intact neutrophils, immortalized fibroblasts and adenocarcinoma cells spontaneously formed DNA-containing extracellular structures during incubation. The process begins with the release of a single DNA-containing strand from the cell immediately after the cells are fixed to the substrate (1 h) and ends after 3 h with the formation of DNA-containing extracellular structures in the form of bundles of parallel fibers. These findings indicate active nature of the secretion of DNA-containing structures by immortalized fibroblasts and HCT116 adenocarcinoma cells.</p>

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Transformed and Tumor Cells Spontaneously Produce DNA-Containing Extracellular Structures In Vitro

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • A. N. Kazimirskii,
  • J. M. Salmasi,
  • G. V. Poryadin,
  • M. I. Panina,
  • A. E. Kim,
  • A. G. Chakhalian

摘要

The parameters of DNA-containing extracellular structures formed by immortalized fibroblasts and HCT116 adenocarcinoma cells during cultivation were studied. Fluorescence microscopy with SYBR Green dye was used to visualize DNA-containing extracellular structures. In contrast to intact neutrophils, immortalized fibroblasts and adenocarcinoma cells spontaneously formed DNA-containing extracellular structures during incubation. The process begins with the release of a single DNA-containing strand from the cell immediately after the cells are fixed to the substrate (1 h) and ends after 3 h with the formation of DNA-containing extracellular structures in the form of bundles of parallel fibers. These findings indicate active nature of the secretion of DNA-containing structures by immortalized fibroblasts and HCT116 adenocarcinoma cells.