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<p>We studied the effect of conditioned media (CM) from cultivated bone marrow stromal cells grown at 10% O<sub>2</sub> on extracellular matrix and vascular component in the healing zone after surgical incision of the uterine wall in Sprague-Dawley rats (<i>n</i>=17). Control group (<i>n</i>=10) received no treatment. On days 5 and 15 after the surgery, the expression of <i>CoL1a1</i>, <i>CoL4a</i>, <i>MMP9</i>, <i>TIMP1</i>, and <i>FGF2</i> genes was evaluated and a morphological study was carried out. On day 5, <i>CoL1a1</i> expression, CD34<sup>+</sup> cell content, and the area of newly formed tissue were lower in the experimental group. On day 15, the expression of <i>MMP9</i>, <i>TIMP1</i>, <i>FGF2</i>, and <i>CoL1a1</i> genes in the control group was lower, while CD34<sup>+</sup> cell content and area of healing zone were higher. Thus, the application of CM reduced the damage area and accelerated the recovery process after surgical full-thickness incision of the uterine wall.</p>

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Morphological and Molecular Peculiarities of Healing of Uterine Incision when Using Conditioned Media of Bone Marrow Stromal Cells Cultivated under Hypoxic Condition

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • N. B. Tikhonova,
  • A. A. Temnov,
  • V. V. Aleksankina,
  • A. P. Aleksankin,
  • T. V. Fokina,
  • A. N. Sklifas,
  • M. G. Aksenova,
  • A. V. Elchaninov,
  • A. P. Milovanov,
  • L. M. Mikhaleva

摘要

We studied the effect of conditioned media (CM) from cultivated bone marrow stromal cells grown at 10% O2 on extracellular matrix and vascular component in the healing zone after surgical incision of the uterine wall in Sprague-Dawley rats (n=17). Control group (n=10) received no treatment. On days 5 and 15 after the surgery, the expression of CoL1a1, CoL4a, MMP9, TIMP1, and FGF2 genes was evaluated and a morphological study was carried out. On day 5, CoL1a1 expression, CD34+ cell content, and the area of newly formed tissue were lower in the experimental group. On day 15, the expression of MMP9, TIMP1, FGF2, and CoL1a1 genes in the control group was lower, while CD34+ cell content and area of healing zone were higher. Thus, the application of CM reduced the damage area and accelerated the recovery process after surgical full-thickness incision of the uterine wall.