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<p>The formation of functional connections between cells derived from neuronal progenitor cells (NPCs) and the developed neuronal network is a crucial task in neurobiology and cellular technologies for medical applications. We developed a technique to co-culture NPCs and mature neuronal cells in a three-chamber microfluidic chip <i>in vitro</i> and studied the features of functional connections among cells derived from NPCs during their integration into a network of differentiated neurons. Starting from day 20 of primary culture development, a network forms from integrated NPCs, which ensures the distribution of bioelectrical activity among neurons in a manner similar to that of differentiated cells.</p>

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Microfluidic Chip for Studying the Mechanisms of Allogeneic Progenitor Neuronal Cells Integration into a Mature Neural Network In Vitro

  • 期刊论文

  • 发表日期：

    2025年1月1日

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  • A. A. Gladkov,
  • M. S. Zemlyanskov,
  • Y. I. Pigareva,
  • V. N. Kolpakov,
  • V. B. Kazantsev,
  • I. V. Mukhina,
  • A. S. Pimashkin

摘要

The formation of functional connections between cells derived from neuronal progenitor cells (NPCs) and the developed neuronal network is a crucial task in neurobiology and cellular technologies for medical applications. We developed a technique to co-culture NPCs and mature neuronal cells in a three-chamber microfluidic chip in vitro and studied the features of functional connections among cells derived from NPCs during their integration into a network of differentiated neurons. Starting from day 20 of primary culture development, a network forms from integrated NPCs, which ensures the distribution of bioelectrical activity among neurons in a manner similar to that of differentiated cells.