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<p>In the present study, the effects of dietary supplementation of different levels of RMP (Reishi mushroom powder) on growth performance, innate immunity, serum biochemistry, immune gene expression, and disease resistance against <i>Plesiomonas shigelloides</i> in Nile tilapia (<i>Oreochromis niloticus</i>) were evaluated using a 60-day feeding trial. The experimental diets were formulated by adding RMP to the basal diet at 0.5%, 1.0%, and 2.0%. Results indicated that fish fed diets containing 1% RMP had significantly improved weight gain and specific growth rate and reduced feed conversion ratio relative to the control group. Compared with the control group, fish fed the 1% RMP diet exhibited significantly lower serum glucose, triglyceride, and cholesterol levels, as well as reduced AST and ALP activities, suggesting potential improvements in overall health status. Serum lysozyme activity was significantly increased in fish fed the 1% RMP diet, whereas respiratory burst activity and superoxide anion production were lower in the RMP-supplemented groups than in the control group. These results indicate that RMP supplementation did not uniformly enhance all measured innate immune parameters. Gene expression analysis showed tissue- and dose-dependent upregulation of immune-related genes (<i>il-1β, il-8, ifn-γ, cc-chemokine</i>, and <i>igm-heavy chain</i>) and antioxidant-related genes (<i>sod</i> and <i>gpx</i>), with the most pronounced responses generally observed in the liver tissue of fish receiving the 1% RMP diet. Following challenge with <i>P. shigelloides</i>, fish fed diets supplemented with 1% RMP exhibited the highest resistance to infection, with a survival rate of 86.7% and a relative percent survival (RPS) value of 73.7%. Overall, dietary supplementation with 1% RMP appeared to be the most effective inclusion level, with beneficial effects on growth performance, selected physiological and immune-related responses, and postchallenge survival in Nile tilapia under the present experimental conditions.</p>

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Effects of dietary Reishi mushroom (Ganoderma lucidum) supplementation on immune response, antioxidant status, and disease resistance in Nile tilapia (Oreochromis niloticus)

  • 期刊论文

  • 发表日期：

    2026年8月1日

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  • Osman Nezih Kenanoğlu,
  • Ebru Yılmaz,
  • Sebahattin Ergün,
  • Sevdan Yılmaz

摘要

In the present study, the effects of dietary supplementation of different levels of RMP (Reishi mushroom powder) on growth performance, innate immunity, serum biochemistry, immune gene expression, and disease resistance against Plesiomonas shigelloides in Nile tilapia (Oreochromis niloticus) were evaluated using a 60-day feeding trial. The experimental diets were formulated by adding RMP to the basal diet at 0.5%, 1.0%, and 2.0%. Results indicated that fish fed diets containing 1% RMP had significantly improved weight gain and specific growth rate and reduced feed conversion ratio relative to the control group. Compared with the control group, fish fed the 1% RMP diet exhibited significantly lower serum glucose, triglyceride, and cholesterol levels, as well as reduced AST and ALP activities, suggesting potential improvements in overall health status. Serum lysozyme activity was significantly increased in fish fed the 1% RMP diet, whereas respiratory burst activity and superoxide anion production were lower in the RMP-supplemented groups than in the control group. These results indicate that RMP supplementation did not uniformly enhance all measured innate immune parameters. Gene expression analysis showed tissue- and dose-dependent upregulation of immune-related genes (il-1β, il-8, ifn-γ, cc-chemokine, and igm-heavy chain) and antioxidant-related genes (sod and gpx), with the most pronounced responses generally observed in the liver tissue of fish receiving the 1% RMP diet. Following challenge with P. shigelloides, fish fed diets supplemented with 1% RMP exhibited the highest resistance to infection, with a survival rate of 86.7% and a relative percent survival (RPS) value of 73.7%. Overall, dietary supplementation with 1% RMP appeared to be the most effective inclusion level, with beneficial effects on growth performance, selected physiological and immune-related responses, and postchallenge survival in Nile tilapia under the present experimental conditions.