Scholarlink

<p>The present study compared the reproductive performance, hormone profile, gene expression, and seed quality of different maternal sizes of Genetically Improved Farmed Tilapia (GIFT, a strain of <i>Oreochromis niloticus</i>) to identify the optimal maternal size for sustainable captive breeding and seed production. The study used 720 healthy brooders of the same age and segregated them into four maternal size groups: 201–400&#xa0;g, 401–600&#xa0;g, 601–800&#xa0;g, and 801–1000&#xa0;g. The brooders were stocked in 12 hapas (60 fish/hapa) at a 2:1 sex ratio (female:male) under triplicate conditions, and the breeding performance of the fish was assessed over a period of 100&#xa0;days. The study recorded significantly higher values for egg volume per female (25.81 ± 0.66&#xa0;mL), total number of eggs/female (3346.00 ± 111.22), diameter of eggs (2.98 ± 0.13&#xa0;mm), and weight of eggs (10.25 ± 0.75&#xa0;mg) in the 801–1000&#xa0;g group followed by the 601–800&#xa0;g group, compared to the other two groups. A significantly higher hatching rate (89.42 ± 0.16% &amp; 88.40 ± 0.19%) was recorded in the 601–800&#xa0;g and 401–600&#xa0;g groups, respectively, when compared with the other two groups. Furthermore, reproductive hormones such as estradiol, progesterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), cortisol, and glucose were measured using radioimmunoassay and enzyme-linked immunosorbent assay kits. The 401–600&#xa0;g maternal group showed significantly lower levels of LH (0.045 ± 0.002 mlU/mL), progesterone (0.079 ± 0.002&#xa0;ng/mL), and estradiol (0.038 ± 0.003&#xa0;ng/mL) than the other three groups. Likewise, significantly higher levels of glucose (147.04 ± 4.33&#xa0;µg/dL) and cortisol (7.50 ± 0.05&#xa0;mg/dL) were observed in the 201–400&#xa0;g size brooders. The expression of key reproductive genes (FSHβ, LHβ, Cyp17, Cyp11, GnRH1, and KISS2) was quantified in brain and gonad samples using quantitative real-time PCR (qRT-PCR). The relative expression of Cyp17 (5.40 ± 0.36), LHβ (9.41 ± 0.12), and KISS2 (9.05 ± 0.07) was significantly upregulated in the 601–800&#xa0;g group compared to the other three groups. In contrast, the expression of Cyp17, LHβ, GnRH1, and KISS2 was downregulated in the 201–400&#xa0;g group compared to the other groups. Further, the seed quality trial found significantly higher body weight gain (220.13 ± 4.83&#xa0;g) in the seeds produced by the maternal size group of 601–800&#xa0;g. Moreover, gene expression analysis of the seeds revealed increased expression of IGF1, GH-R, and MyoD in the seeds produced by the 601–800&#xa0;g group compared to the other maternal groups. Overall, the results of the present study suggest that a brooder size of 601–800&#xa0;g can be used by GIFT tilapia hatcheries to produce maximum reproductive output with better-quality seeds.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Maternal size affects the reproductive traits, hormones, gene expression, and seed quality traits in genetically improved farmed tilapia

  • 期刊论文

  • 发表日期：

    2026年7月8日

查看全文
  • Venkatesan Vanathi,
  • Cheryl Antony,
  • Anand S.,
  • George Rayventh C.,
  • Parameswaran Hariharan,
  • Somu Sunder Lingam R.

摘要

The present study compared the reproductive performance, hormone profile, gene expression, and seed quality of different maternal sizes of Genetically Improved Farmed Tilapia (GIFT, a strain of Oreochromis niloticus) to identify the optimal maternal size for sustainable captive breeding and seed production. The study used 720 healthy brooders of the same age and segregated them into four maternal size groups: 201–400 g, 401–600 g, 601–800 g, and 801–1000 g. The brooders were stocked in 12 hapas (60 fish/hapa) at a 2:1 sex ratio (female:male) under triplicate conditions, and the breeding performance of the fish was assessed over a period of 100 days. The study recorded significantly higher values for egg volume per female (25.81 ± 0.66 mL), total number of eggs/female (3346.00 ± 111.22), diameter of eggs (2.98 ± 0.13 mm), and weight of eggs (10.25 ± 0.75 mg) in the 801–1000 g group followed by the 601–800 g group, compared to the other two groups. A significantly higher hatching rate (89.42 ± 0.16% & 88.40 ± 0.19%) was recorded in the 601–800 g and 401–600 g groups, respectively, when compared with the other two groups. Furthermore, reproductive hormones such as estradiol, progesterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), cortisol, and glucose were measured using radioimmunoassay and enzyme-linked immunosorbent assay kits. The 401–600 g maternal group showed significantly lower levels of LH (0.045 ± 0.002 mlU/mL), progesterone (0.079 ± 0.002 ng/mL), and estradiol (0.038 ± 0.003 ng/mL) than the other three groups. Likewise, significantly higher levels of glucose (147.04 ± 4.33 µg/dL) and cortisol (7.50 ± 0.05 mg/dL) were observed in the 201–400 g size brooders. The expression of key reproductive genes (FSHβ, LHβ, Cyp17, Cyp11, GnRH1, and KISS2) was quantified in brain and gonad samples using quantitative real-time PCR (qRT-PCR). The relative expression of Cyp17 (5.40 ± 0.36), LHβ (9.41 ± 0.12), and KISS2 (9.05 ± 0.07) was significantly upregulated in the 601–800 g group compared to the other three groups. In contrast, the expression of Cyp17, LHβ, GnRH1, and KISS2 was downregulated in the 201–400 g group compared to the other groups. Further, the seed quality trial found significantly higher body weight gain (220.13 ± 4.83 g) in the seeds produced by the maternal size group of 601–800 g. Moreover, gene expression analysis of the seeds revealed increased expression of IGF1, GH-R, and MyoD in the seeds produced by the 601–800 g group compared to the other maternal groups. Overall, the results of the present study suggest that a brooder size of 601–800 g can be used by GIFT tilapia hatcheries to produce maximum reproductive output with better-quality seeds.