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<p>The mandarin fish (<i>Siniperca chuatsi</i>) is a high-value carnivorous species whose exclusive reliance on live prey hinders sustainable aquaculture. To uncover the systemic adaptation mechanisms to formulated feeds, we conducted a longitudinal study analyzing the liver and gut transcriptomes across a 30-day feeding domestication protocol. Fish were transitioned through three phases: initial feeding with live feed (LF), a transitional period with a dead-fish mixture (DM), and final feeding with a complete artificial diet (AD). Comparative transcriptomics and weighted gene co-expression network analysis (WGCNA) revealed distinct, stage-specific responses. During the initial transition (LF vs. DM), a coordinated stress response was activated across the gut-liver axis, characterized by up-regulation of transcription factors (e.g., <i>jun</i>, <i>fosab</i>) in pathways like MAPK and Toll-like receptor signaling. In contrast, successful adaptation to the AD diet (AD vs. DM) involved a shift toward metabolic reprogramming. The gut enhanced lipid metabolism capacity (e.g., up-regulation of <i>fads2</i>, <i>fabp2</i>), while the liver attenuated inflammatory stress (e.g., down-regulation of MAPK pathway genes). WGCNA further confirmed this functional specialization, showing key gene modules shifted from stress/immune responses to nutrient metabolism and homeostasis. Our findings demonstrate that dietary domestication in mandarin fish is achieved through temporally coordinated and organ-specialized adaptations within the gut-liver axis, providing crucial molecular insights for improving feed acceptance in this species.</p>

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Transcriptomic analysis reveals organ-specific adaptations in the gut-liver axis during diet domestication of mandarin fish (Siniperca chuatsi)

  • 期刊论文

  • 发表日期：

    2026年7月8日

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  • Hao-Yu Li,
  • Zhi-Guang Hou,
  • Yi-Huan Xu,
  • Cheng-Bin Wu,
  • Zun-Li Qi,
  • Xiao-Wei Gao,
  • Chun‑Long Zhao,
  • Xin Zhao,
  • Li-Han Zhang

摘要

The mandarin fish (Siniperca chuatsi) is a high-value carnivorous species whose exclusive reliance on live prey hinders sustainable aquaculture. To uncover the systemic adaptation mechanisms to formulated feeds, we conducted a longitudinal study analyzing the liver and gut transcriptomes across a 30-day feeding domestication protocol. Fish were transitioned through three phases: initial feeding with live feed (LF), a transitional period with a dead-fish mixture (DM), and final feeding with a complete artificial diet (AD). Comparative transcriptomics and weighted gene co-expression network analysis (WGCNA) revealed distinct, stage-specific responses. During the initial transition (LF vs. DM), a coordinated stress response was activated across the gut-liver axis, characterized by up-regulation of transcription factors (e.g., jun, fosab) in pathways like MAPK and Toll-like receptor signaling. In contrast, successful adaptation to the AD diet (AD vs. DM) involved a shift toward metabolic reprogramming. The gut enhanced lipid metabolism capacity (e.g., up-regulation of fads2, fabp2), while the liver attenuated inflammatory stress (e.g., down-regulation of MAPK pathway genes). WGCNA further confirmed this functional specialization, showing key gene modules shifted from stress/immune responses to nutrient metabolism and homeostasis. Our findings demonstrate that dietary domestication in mandarin fish is achieved through temporally coordinated and organ-specialized adaptations within the gut-liver axis, providing crucial molecular insights for improving feed acceptance in this species.