Scholarlink

<p>Lysophosphatidylcholine (LPC) is a key phospholipid intermediate with roles in membrane remodeling, lipid transport, and metabolic signaling; yet its direct physiological effects at different doses remain poorly understood in crustaceans. Here, we evaluated the dose-dependent effects of intramuscular LPC injection (0.04 and 0.08&#xa0;mg/g body weight) on hepatopancreatic metabolism, antioxidant capacity, and immune response in <i>Macrobrachium rosenbergii</i> using non-targeted metabolomics, biochemical assays, and gene expression analysis. Low-dose LPC mainly affected carbohydrate, amino acid, and energy metabolism pathways, contributing to basic energy balance, whereas high-dose LPC enriched lipid degradation and glutathione pathways, indicating enhanced metabolic turnover. Gene expression analysis revealed clear lipid metabolic differentiation: low-dose LPC significantly upregulated carnitine palmitoyltransferase 2 (<i>CPT2</i>), related to fatty acid <i>β</i>-oxidation, whereas high-dose LPC upregulated fatty acid synthase (<i>FAS</i>), suggesting a shift towards lipid metabolism. The low-dose maintained a stable blood glucose level, whereas the high-dose caused a transient early increase, suggesting enhanced energy mobilization and lipid turnover. Both doses significantly increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) while reducing malondialdehyde (MDA) levels. Low-dose LPC was more effective in elevating CAT and GSH-Px, while the high-dose enhanced SOD activity. In summary, low-dose LPC primarily maintained homeostasis by promoting fatty acid oxidation and energy balance, while high-dose LPC enhanced lipid synthesis, energy mobilization, and physiological robustness. Short-term LPC injection produced clear dose-dependent improvements in metabolic, antioxidant, and immune functions in <i>M. rosenbergii</i>, supporting its potential as a functional additive in sustainable aquaculture.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metabolic and antioxidant responses to short-term lysophosphatidylcholine injection in Macrobrachium rosenbergii

  • 期刊论文

  • 发表日期：

    2026年7月9日

查看全文
  • Zongsheng Qiu,
  • Jianle Yang,
  • Xinyi Wei,
  • Yuqiang Wei,
  • Shasha Liu,
  • Jianhua Zhao,
  • Qiyou Xu,
  • Clement R. de Cruz

摘要

Lysophosphatidylcholine (LPC) is a key phospholipid intermediate with roles in membrane remodeling, lipid transport, and metabolic signaling; yet its direct physiological effects at different doses remain poorly understood in crustaceans. Here, we evaluated the dose-dependent effects of intramuscular LPC injection (0.04 and 0.08 mg/g body weight) on hepatopancreatic metabolism, antioxidant capacity, and immune response in Macrobrachium rosenbergii using non-targeted metabolomics, biochemical assays, and gene expression analysis. Low-dose LPC mainly affected carbohydrate, amino acid, and energy metabolism pathways, contributing to basic energy balance, whereas high-dose LPC enriched lipid degradation and glutathione pathways, indicating enhanced metabolic turnover. Gene expression analysis revealed clear lipid metabolic differentiation: low-dose LPC significantly upregulated carnitine palmitoyltransferase 2 (CPT2), related to fatty acid β-oxidation, whereas high-dose LPC upregulated fatty acid synthase (FAS), suggesting a shift towards lipid metabolism. The low-dose maintained a stable blood glucose level, whereas the high-dose caused a transient early increase, suggesting enhanced energy mobilization and lipid turnover. Both doses significantly increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) while reducing malondialdehyde (MDA) levels. Low-dose LPC was more effective in elevating CAT and GSH-Px, while the high-dose enhanced SOD activity. In summary, low-dose LPC primarily maintained homeostasis by promoting fatty acid oxidation and energy balance, while high-dose LPC enhanced lipid synthesis, energy mobilization, and physiological robustness. Short-term LPC injection produced clear dose-dependent improvements in metabolic, antioxidant, and immune functions in M. rosenbergii, supporting its potential as a functional additive in sustainable aquaculture.