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<p>The aim of this study was to investigate the dietary zinc (Zn, from ZnSO₄·H₂O) requirement of subadult red swamp crayfish (<i>Procambarus clarkii</i>) through a feeding trial followed by the analysis of growth performance, immune status, antioxidant activity, and lipid profile. Red swamp crayfish were fed six isonitrogenous and isocaloric diets, containing different Zn content of 45.58, 50.33, 58.82, 68.29, 94.11, and 142.72 mg/kg for 9 weeks. The results showed that, compared with the control group, when dietary Zn content reached 68.29 mg/kg, red swamp crayfish exhibited a significant increase in final body weight (FBW), feeding rate (FR), weight gain rate (WGR), and specific growth rate (SGR), along with a significant reduction in feed conversion ratio (FCR) (<i>P</i> &lt; 0.05). As dietary Zn levels rose, Zn content in whole crayfish and crayfish shells increased first and then reached a plateau when the dietary Zn content reached 94.11 mg/kg (<i>P</i> &lt; 0.05). In contrast, the content of iron, copper, and manganese in whole crayfish and crayfish shell, as well as iron content in muscle, initially decreased and then stabilized with increasing dietary Zn levels (<i>P</i> &lt; 0.05). Compared with the control group, a dietary Zn content of 68.29 mg/kg significantly enhanced serum immune capacity and hepatopancreatic antioxidant activities (<i>P</i> &lt; 0.05), significantly improved hepatocyte health and reduced lipid accumulation (<i>P</i> &lt; 0.05), significantly downregulated the expression of <i>fasn</i>, <i>acc</i>, <i>acsl</i>, and <i>srebp</i> in the hepatopancreas (<i>P</i> &lt; 0.05), and significantly upregulated the expression of <i>cpt2</i> and <i>acox1</i> in the hepatopancreas (<i>P</i> &lt; 0.05). Based on the broken-line analysis of SGR, FCR, and immune enzyme AKP activity, the optimal dietary Zn requirement for subadult red swamp crayfish was assessed to be 66.07, 65.60, and 66.13&#xa0;mg/kg, respectively, using ZnSO₄·H₂O as the Zn source. For practical feed formulations, a Zn requirement of 66 mg/kg diet is recommended to support optimal growth and health for subadult red swamp crayfish.</p>

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Quantifying the dietary zinc requirement for subadult red swamp crayfish (Procambarus clarkii) based on growth performance and health status

  • 期刊论文

  • 发表日期：

    2026年6月2日

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  • Huimin Tan,
  • Pengyan Yang,
  • Liang Wang,
  • Shouqi Xie,
  • Xiaofang Zhu,
  • Wenhuan Zhu,
  • Qingsong Tan

摘要

The aim of this study was to investigate the dietary zinc (Zn, from ZnSO₄·H₂O) requirement of subadult red swamp crayfish (Procambarus clarkii) through a feeding trial followed by the analysis of growth performance, immune status, antioxidant activity, and lipid profile. Red swamp crayfish were fed six isonitrogenous and isocaloric diets, containing different Zn content of 45.58, 50.33, 58.82, 68.29, 94.11, and 142.72 mg/kg for 9 weeks. The results showed that, compared with the control group, when dietary Zn content reached 68.29 mg/kg, red swamp crayfish exhibited a significant increase in final body weight (FBW), feeding rate (FR), weight gain rate (WGR), and specific growth rate (SGR), along with a significant reduction in feed conversion ratio (FCR) (P < 0.05). As dietary Zn levels rose, Zn content in whole crayfish and crayfish shells increased first and then reached a plateau when the dietary Zn content reached 94.11 mg/kg (P < 0.05). In contrast, the content of iron, copper, and manganese in whole crayfish and crayfish shell, as well as iron content in muscle, initially decreased and then stabilized with increasing dietary Zn levels (P < 0.05). Compared with the control group, a dietary Zn content of 68.29 mg/kg significantly enhanced serum immune capacity and hepatopancreatic antioxidant activities (P < 0.05), significantly improved hepatocyte health and reduced lipid accumulation (P < 0.05), significantly downregulated the expression of fasn, acc, acsl, and srebp in the hepatopancreas (P < 0.05), and significantly upregulated the expression of cpt2 and acox1 in the hepatopancreas (P < 0.05). Based on the broken-line analysis of SGR, FCR, and immune enzyme AKP activity, the optimal dietary Zn requirement for subadult red swamp crayfish was assessed to be 66.07, 65.60, and 66.13 mg/kg, respectively, using ZnSO₄·H₂O as the Zn source. For practical feed formulations, a Zn requirement of 66 mg/kg diet is recommended to support optimal growth and health for subadult red swamp crayfish.