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<p>Bacterial diseases have increasingly become a major factor limiting the development of yellowfin tuna (<i>Thunnus albacares</i>) aquaculture. Although yellowfin tuna is of considerable economic importance in aquaculture, pathological studies on cultured juveniles remain limited. In the present study, juvenile yellowfin tuna showing clinical signs of infection were collected during a spontaneous disease outbreak at an indoor yellowfin tuna aquaculture facility in Lingshui, Hainan Province, China. To identify the causative pathogens and determine their tissue-specific bacterial loads and associated histopathological damage, bacterial isolation, 16S rDNA sequence analysis, and species-specific gene sequencing were performed. The results identified <i>Vibrio harveyi</i> and <i>Vibrio parahaemolyticus</i> as the predominant pathogenic bacteria. Quantitative real-time PCR analysis showed distinct tissue tropism between the two species: <i>V. harveyi</i> exhibited the highest bacterial loads in the intestine and spleen, whereas <i>V. parahaemolyticus</i> maintained relatively high loads in the intestine, gills, liver, and kidney (<i>P</i> &lt; 0.05). Histopathological examination revealed severe multi-organ damage in diseased fish, including intestinal villus atrophy, disorganized hepatic cell arrangement, and lamellar fusion in the gills, accompanied by marked inflammatory cell infiltration. These findings provide an important scientific basis for the early diagnosis and integrated control of bacterial diseases in cultured juvenile yellowfin tuna.</p>

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Integrated identification, quantification, and histopathological characterization of Vibrio spp. associated with disease in juvenile yellowfin tuna (Thunnus albacares)

  • 期刊论文

  • 发表日期：

    2026年6月1日

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  • Yuzhuo You,
  • Zhengyi Fu,
  • Tao Zhang,
  • Jing Hu,
  • Zhenhua Ma,
  • Gang Yu

摘要

Bacterial diseases have increasingly become a major factor limiting the development of yellowfin tuna (Thunnus albacares) aquaculture. Although yellowfin tuna is of considerable economic importance in aquaculture, pathological studies on cultured juveniles remain limited. In the present study, juvenile yellowfin tuna showing clinical signs of infection were collected during a spontaneous disease outbreak at an indoor yellowfin tuna aquaculture facility in Lingshui, Hainan Province, China. To identify the causative pathogens and determine their tissue-specific bacterial loads and associated histopathological damage, bacterial isolation, 16S rDNA sequence analysis, and species-specific gene sequencing were performed. The results identified Vibrio harveyi and Vibrio parahaemolyticus as the predominant pathogenic bacteria. Quantitative real-time PCR analysis showed distinct tissue tropism between the two species: V. harveyi exhibited the highest bacterial loads in the intestine and spleen, whereas V. parahaemolyticus maintained relatively high loads in the intestine, gills, liver, and kidney (P < 0.05). Histopathological examination revealed severe multi-organ damage in diseased fish, including intestinal villus atrophy, disorganized hepatic cell arrangement, and lamellar fusion in the gills, accompanied by marked inflammatory cell infiltration. These findings provide an important scientific basis for the early diagnosis and integrated control of bacterial diseases in cultured juvenile yellowfin tuna.