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<p>The aim of this study was to evaluate Viligen® NE (Alltech®, USA) as a feed additive for <i>Penaeus vannamei</i> reared in a biofloc system during the nursery phase, focusing on growth performance, intestinal microbiological analysis, and resistance tocold challenge. Shrimp post-larvae (41&#xa0;mg) were fed for 6 weeks with four experimental diets: (a) 0.5&#xa0;g Viligen® NE kg⁻<sup>1</sup>, (b) 1&#xa0;g Viligen® NE kg⁻<sup>1</sup>, (c) 2&#xa0;g Viligen® NE kg⁻<sup>1</sup>, and (d) a control diet (0&#xa0;g Viligen® NE kg⁻<sup>1</sup>). The experiment was conducted in quadruplicate, totaling 16 experimental tanks, with a stocking density of 2000 post-larvae m⁻<sup>3</sup>. Shrimp growth performance parameters were positively influenced by the inclusion of Viligen® NE in the diet. Polynomial regression indicated an optimal inclusion level of 1.17&#xa0;g&#xa0;kg⁻<sup>1</sup> for maximizing specific growth rate and weight gain. Additionally, supplementation at 1&#xa0;g&#xa0;kg⁻<sup>1</sup> enhanced shrimp resistance to cold challenge. However, no significant differences were observed in intestinal counts of total heterotrophic bacteria or <i>Vibrio</i> spp. among treatments. In conclusion, dietary supplementation with Viligen® NE at an optimal level of 1.17&#xa0;g&#xa0;kg⁻<sup>1</sup> improved growth performance, and 1&#xa0;g&#xa0;kg⁻<sup>1</sup> enhanced cold challenge resistance of nursery-phase shrimp reared in a biofloc system.</p>

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Evaluation of Viligen® NE as a feed additive for Pacific white shrimp reared in a biofloc system during the nursery phase

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月6日

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  • Camilla Souza Miranda,
  • Ivanilson de Lima Santos,
  • Ramon Felipe Siqueira Carneiro,
  • Isabela Claudiana Pinheiro,
  • Norha Constanza Bolívar,
  • Débora Machado Fracalossi,
  • Philip Lyons,
  • Anne Koontz,
  • Scheila Anelise Pereira Dutra,
  • Felipe Boéchat Vieira

摘要

The aim of this study was to evaluate Viligen® NE (Alltech®, USA) as a feed additive for Penaeus vannamei reared in a biofloc system during the nursery phase, focusing on growth performance, intestinal microbiological analysis, and resistance tocold challenge. Shrimp post-larvae (41 mg) were fed for 6 weeks with four experimental diets: (a) 0.5 g Viligen® NE kg⁻1, (b) 1 g Viligen® NE kg⁻1, (c) 2 g Viligen® NE kg⁻1, and (d) a control diet (0 g Viligen® NE kg⁻1). The experiment was conducted in quadruplicate, totaling 16 experimental tanks, with a stocking density of 2000 post-larvae m⁻3. Shrimp growth performance parameters were positively influenced by the inclusion of Viligen® NE in the diet. Polynomial regression indicated an optimal inclusion level of 1.17 g kg⁻1 for maximizing specific growth rate and weight gain. Additionally, supplementation at 1 g kg⁻1 enhanced shrimp resistance to cold challenge. However, no significant differences were observed in intestinal counts of total heterotrophic bacteria or Vibrio spp. among treatments. In conclusion, dietary supplementation with Viligen® NE at an optimal level of 1.17 g kg⁻1 improved growth performance, and 1 g kg⁻1 enhanced cold challenge resistance of nursery-phase shrimp reared in a biofloc system.