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<p>Lipid metabolism disorders induced by high-fat diets represent a common issue in the farming of spotted sea bass (<i>Lateolabrax maculatus</i>). Chlorogenic acid (CGA), a phenolic acid commonly extracted from plants such as <i>Lonicera japonica</i> or <i>Eucommia ulmoides</i>, has demonstrated potential in improving lipid metabolic disorders in humans and various aquatic species. This study aimed to document the hepatic transcriptomic and metabolic profiling of spotted sea bass in response to chlorogenic acid supplementation under a high-fat diet. Fish were fed either a high-fat diet (HFD) or a similar diet, but supplemented with 300&#xa0;mg/kg CGA. The fish fed with the two types of feed showed no mortality and comparable growth performance, but the CGA group exhibited significantly reduced blood lipid levels and hepatic fat deposition. Transcriptomic analysis revealed 118 upregulated and 68 downregulated differentially expressed genes (DEGs). KEGG enrichment indicated that these DEGs were primarily associated with inflammatory responses and amino acid metabolism pathways. Notably, the upregulated genes <i>fatp</i>, <i>cpt-1</i>, and <i>cyp7a1</i> in the PPAR signaling pathway were implicated in fatty acid transport, β-oxidation, and cholesterol conversion to bile acids, respectively. In the ferroptosis pathways, downregulation of <i>ho-1</i> expression contributed to altered lipid peroxidation processes. Results identified 243 upregulated and 291 downregulated differential lipid molecules (DLMs). KEGG enrichment also demonstrated that DLMs were predominantly enriched in metabolic pathways related to glycerophospholipids, fatty acids, steroids, and steroid derivatives. In summary, chlorogenic acid modulates immune responses, lipid metabolism, and protein metabolism. These findings establish a foundation for further research on CGA’s functional roles to advance sustainable spotted sea bass aquaculture practices.</p>

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Hepatic transcriptomic and metabolic profiling of spotted sea bass (Lateolabrax maculatus) in response to chlorogenic acid supplementation under high-fat diet

  • 期刊论文

  • 发表日期：

    2026年1月14日

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  • Jianrong Ma,
  • Anle Xu,
  • Sishun Zhou,
  • Lumin Kong,
  • Hao Lin,
  • Zhongbao Li

摘要

Lipid metabolism disorders induced by high-fat diets represent a common issue in the farming of spotted sea bass (Lateolabrax maculatus). Chlorogenic acid (CGA), a phenolic acid commonly extracted from plants such as Lonicera japonica or Eucommia ulmoides, has demonstrated potential in improving lipid metabolic disorders in humans and various aquatic species. This study aimed to document the hepatic transcriptomic and metabolic profiling of spotted sea bass in response to chlorogenic acid supplementation under a high-fat diet. Fish were fed either a high-fat diet (HFD) or a similar diet, but supplemented with 300 mg/kg CGA. The fish fed with the two types of feed showed no mortality and comparable growth performance, but the CGA group exhibited significantly reduced blood lipid levels and hepatic fat deposition. Transcriptomic analysis revealed 118 upregulated and 68 downregulated differentially expressed genes (DEGs). KEGG enrichment indicated that these DEGs were primarily associated with inflammatory responses and amino acid metabolism pathways. Notably, the upregulated genes fatp, cpt-1, and cyp7a1 in the PPAR signaling pathway were implicated in fatty acid transport, β-oxidation, and cholesterol conversion to bile acids, respectively. In the ferroptosis pathways, downregulation of ho-1 expression contributed to altered lipid peroxidation processes. Results identified 243 upregulated and 291 downregulated differential lipid molecules (DLMs). KEGG enrichment also demonstrated that DLMs were predominantly enriched in metabolic pathways related to glycerophospholipids, fatty acids, steroids, and steroid derivatives. In summary, chlorogenic acid modulates immune responses, lipid metabolism, and protein metabolism. These findings establish a foundation for further research on CGA’s functional roles to advance sustainable spotted sea bass aquaculture practices.