Scholarlink

<p>The adoption of high-fat diets (HFD) in aquaculture, while economically beneficial, often induces hepatic injury, posing a major challenge to farmed fish like <i>Monopterus albus</i>. This study aimed to investigate the potential of dietary soy isoflavones (SIF) to alleviate HFD-induced liver damage by concurrently modulating lipid metabolism, oxidative stress, endoplasmic reticulum stress (ERS), and inflammation. Four isonitrogenous diets were formulated: a control diet (CON, 5.96% lipid), a high-fat diet (HFD, 11.96% lipid), and the HFD supplemented with 50 or 100&#xa0;mg/kg SIF (HSIF50 and HSIF100 groups, respectively). Following an 8-week feeding trial, the HFD group exhibited significantly impaired growth performance and intestinal digestive enzyme activity, alongside severe hepatic steatosis. This was driven by a marked dysregulation of hepatic lipid metabolism, evidenced by upregulated lipogenic genes (<i>srebp1</i>, <i>fas</i>) and downregulated β-oxidation genes (<i>aco</i>, <i>cpt1</i>). Furthermore, HFD feeding triggered substantial hepatic injury, characterized by exacerbated vacuolization, diminished antioxidant capacity (reduced SOD and CAT activities), induced ERS (elevated <i>grp78</i> and <i>atf6</i>), and activation of pro-inflammatory cytokines (upregulated <i>il1β</i> and <i>tnfα</i>). Dietary SIF supplementation, particularly at 50&#xa0;mg/kg, effectively mitigated these adverse effects by restoring lipid metabolic balance, enhancing antioxidant defenses, alleviating ERS, and suppressing inflammatory responses. These results demonstrate that SIF confers hepatoprotection in <i>M</i>. <i>albus</i> by targeting the integrated pathway of metabolic and cellular stress, with an optimal dose of 50&#xa0;mg/kg. Further studies should explore the long-term effects and molecular mechanisms underlying SIF-mediated protection in aquatic species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Soy isoflavone alleviates high-fat diet-induced liver injury in rice field eel (Monopterus albus) by ameliorating lipid metabolic disorders, oxidative and endoplasmic reticulum stress, and inflammation

  • 期刊论文

  • 发表日期：

    2025年12月11日

查看全文
  • Quan Li,
  • Huahong Wei,
  • Tao Zhou,
  • Kai Xie,
  • Yi Hu,
  • Junzhi Zhang

摘要

The adoption of high-fat diets (HFD) in aquaculture, while economically beneficial, often induces hepatic injury, posing a major challenge to farmed fish like Monopterus albus. This study aimed to investigate the potential of dietary soy isoflavones (SIF) to alleviate HFD-induced liver damage by concurrently modulating lipid metabolism, oxidative stress, endoplasmic reticulum stress (ERS), and inflammation. Four isonitrogenous diets were formulated: a control diet (CON, 5.96% lipid), a high-fat diet (HFD, 11.96% lipid), and the HFD supplemented with 50 or 100 mg/kg SIF (HSIF50 and HSIF100 groups, respectively). Following an 8-week feeding trial, the HFD group exhibited significantly impaired growth performance and intestinal digestive enzyme activity, alongside severe hepatic steatosis. This was driven by a marked dysregulation of hepatic lipid metabolism, evidenced by upregulated lipogenic genes (srebp1, fas) and downregulated β-oxidation genes (aco, cpt1). Furthermore, HFD feeding triggered substantial hepatic injury, characterized by exacerbated vacuolization, diminished antioxidant capacity (reduced SOD and CAT activities), induced ERS (elevated grp78 and atf6), and activation of pro-inflammatory cytokines (upregulated il1β and tnfα). Dietary SIF supplementation, particularly at 50 mg/kg, effectively mitigated these adverse effects by restoring lipid metabolic balance, enhancing antioxidant defenses, alleviating ERS, and suppressing inflammatory responses. These results demonstrate that SIF confers hepatoprotection in M. albus by targeting the integrated pathway of metabolic and cellular stress, with an optimal dose of 50 mg/kg. Further studies should explore the long-term effects and molecular mechanisms underlying SIF-mediated protection in aquatic species.