Scholarlink

<p>The Chinese soft-shelled turtle (<i>Pelodiscus sinensis</i>) is a commercially important aquaculture species in China. The intestine serves as a primary organ for digestion and nutrient absorption, as well as a critical barrier against harmful external substances. <i>Aeromonas hydrophila</i>, a common pathogenic bacterium in aquaculture, causes various diseases in <i>P. sinensis</i>, leading to metabolic alterations and tissue damage in organs. In this study, we investigated the transcriptional responses of intestinal injury induced by <i>A. hydrophila</i> infection in <i>P. sinensis</i>. The results showed that infection led to severe morphological damage, including atrophy of intestinal villi and significant reductions in villus height, width, and crypt depth in the anterior intestine. TUNEL staining revealed a highly significant increase in late apoptotic cells in the infected group compared to the control group. Furthermore, <i>A. hydrophila</i> infection significantly up-regulated the expression of pro-apoptotic genes. Transcriptome analysis identified 345 significant DEGs (183 up-regulated, 162 down-regulated) in the intestine of <i>P. sinensis</i> following <i>A. hydrophila</i> infection. Among the most significantly altered genes, those related to immunity and apoptosis were strongly up-regulated, whereas genes involved in epithelial integrity and digestion were markedly down-regulated. GO enrichment revealed significant terms associated with response to biotic stimulus, cell adhesion, and cytoskeleton organization, while KEGG analysis highlighted tight junction, glycosaminoglycan degradation, metabolic pathways, and lysosome as the most significantly enriched core pathways. These results collectively indicated profound alterations in immune response, barrier function, and metabolic homeostasis during bacterial infection.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptome reveals the molecular response of the Chinese soft-shelled turtle (Pelodiscus sinensis) intestine to Aeromonas hydrophila infection

  • 期刊论文

  • 发表日期：

    2025年11月26日

查看全文
  • Haoran Song,
  • Juntao Zhu,
  • Qianqian Huang,
  • Yukai Zhu,
  • Chengjie Gao,
  • Caiyan Li,
  • Jiaxiang Luo

摘要

The Chinese soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquaculture species in China. The intestine serves as a primary organ for digestion and nutrient absorption, as well as a critical barrier against harmful external substances. Aeromonas hydrophila, a common pathogenic bacterium in aquaculture, causes various diseases in P. sinensis, leading to metabolic alterations and tissue damage in organs. In this study, we investigated the transcriptional responses of intestinal injury induced by A. hydrophila infection in P. sinensis. The results showed that infection led to severe morphological damage, including atrophy of intestinal villi and significant reductions in villus height, width, and crypt depth in the anterior intestine. TUNEL staining revealed a highly significant increase in late apoptotic cells in the infected group compared to the control group. Furthermore, A. hydrophila infection significantly up-regulated the expression of pro-apoptotic genes. Transcriptome analysis identified 345 significant DEGs (183 up-regulated, 162 down-regulated) in the intestine of P. sinensis following A. hydrophila infection. Among the most significantly altered genes, those related to immunity and apoptosis were strongly up-regulated, whereas genes involved in epithelial integrity and digestion were markedly down-regulated. GO enrichment revealed significant terms associated with response to biotic stimulus, cell adhesion, and cytoskeleton organization, while KEGG analysis highlighted tight junction, glycosaminoglycan degradation, metabolic pathways, and lysosome as the most significantly enriched core pathways. These results collectively indicated profound alterations in immune response, barrier function, and metabolic homeostasis during bacterial infection.