Scholarlink

<p>Due to the ban on antibiotics in aquaculture, there is a growing interest in using immunomodulatory agents to support the production and health of Tilapia. Hesperidin (HP), a bioactive flavonoid with multiple health benefits. This study analyzed the dose-dependent effect of dietary hesperidin on tilapia’s blood biomarkers, antioxidant status, growth performance, immune/inflammatory response, and resistance to <i>Fusarium oxysporum</i> (Faus-ox) infection. A total of 240 tilapia were split into four groups (60 fish/group) and fed diets containing 200, 100, 50, or 0 mg HP/kg diet for 56 days. Findings indicate that the addition of 200 or 100 mg/kg of HP significantly enhanced the growth indices compared with different groups (linear effect, <i>P</i> &lt; 0.05). However, feed intake was the greatest in HP100 treatment compared to the other treatments (linear effect, <i>P</i> &lt; 0.05). All HP-supplemented treatments showed enhanced protein and moisture content while simultaneously exhibiting a decrease in ash and lipid content (<i>P</i> &lt; 0.05, linear effect). The addition of HP caused a significant, dose-dependent reduction in liver enzymes (except GGT) and creatinine levels. Furthermore, digestive enzymes (amylase, lipase and protease) were the highest in the HP200 treatment relative to the non-addition diet (<i>P</i> &lt; 0.05, quadratic effect). The addition of HP improved antioxidant status, as evidenced by higher rates of GPx and TAC in HP100 and HP200 treatments compared to others (quadratic impact, <i>P</i> &lt; 0.05). Furthermore, oxidative stress markers specifically PC (linear impact, <i>P</i> &lt; 0.05) and MDA (quadratic impact, <i>P</i> &lt; 0.001) were significantly reduced across all HP treatment groups. Dietary HP supplementation significantly enhanced humoral immunity, as shown by increased levels of IgM, lysozyme, and IgG activity (<i>P</i> &lt; 0.05). Furthermore, serum TNF-α and TRL4 levels exhibited a quadratic response to the HP diets (<i>P</i> &lt; 0.01). When HP was added to the diet, the fish’s resistance to <i>Fusarium oxysporum</i> was higher than when they were fed a control diet. Overall, the addition of hesperidin improved growth performance, immunity, and antioxidant status, while simultaneously reducing inflammatory and oxidative stress markers. It also enhanced resistance to <i>Fusarium oxysporum</i> infection, supporting the sustainability of tilapia fish farming.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Analysis of the dose–response dietary effects of hesperidin on growth, blood biomarkers, antioxidant homeostasis, immune/inflammatory signaling, and resistance to Fusarium oxysporum infection in Oreochromis niloticus (Nile Tilapia)

  • 期刊论文

  • 发表日期：

    2025年11月21日

查看全文
  • Najah M. Albaqami,
  • Amera N. Alqahtani

摘要

Due to the ban on antibiotics in aquaculture, there is a growing interest in using immunomodulatory agents to support the production and health of Tilapia. Hesperidin (HP), a bioactive flavonoid with multiple health benefits. This study analyzed the dose-dependent effect of dietary hesperidin on tilapia’s blood biomarkers, antioxidant status, growth performance, immune/inflammatory response, and resistance to Fusarium oxysporum (Faus-ox) infection. A total of 240 tilapia were split into four groups (60 fish/group) and fed diets containing 200, 100, 50, or 0 mg HP/kg diet for 56 days. Findings indicate that the addition of 200 or 100 mg/kg of HP significantly enhanced the growth indices compared with different groups (linear effect, P < 0.05). However, feed intake was the greatest in HP100 treatment compared to the other treatments (linear effect, P < 0.05). All HP-supplemented treatments showed enhanced protein and moisture content while simultaneously exhibiting a decrease in ash and lipid content (P < 0.05, linear effect). The addition of HP caused a significant, dose-dependent reduction in liver enzymes (except GGT) and creatinine levels. Furthermore, digestive enzymes (amylase, lipase and protease) were the highest in the HP200 treatment relative to the non-addition diet (P < 0.05, quadratic effect). The addition of HP improved antioxidant status, as evidenced by higher rates of GPx and TAC in HP100 and HP200 treatments compared to others (quadratic impact, P < 0.05). Furthermore, oxidative stress markers specifically PC (linear impact, P < 0.05) and MDA (quadratic impact, P < 0.001) were significantly reduced across all HP treatment groups. Dietary HP supplementation significantly enhanced humoral immunity, as shown by increased levels of IgM, lysozyme, and IgG activity (P < 0.05). Furthermore, serum TNF-α and TRL4 levels exhibited a quadratic response to the HP diets (P < 0.01). When HP was added to the diet, the fish’s resistance to Fusarium oxysporum was higher than when they were fed a control diet. Overall, the addition of hesperidin improved growth performance, immunity, and antioxidant status, while simultaneously reducing inflammatory and oxidative stress markers. It also enhanced resistance to Fusarium oxysporum infection, supporting the sustainability of tilapia fish farming.