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<p>Microbes pose a central role in successful management of recirculating aquaculture systems (RAS). As a result, the need to monitor and quantify the planktonic and surface-oriented microorganisms is becoming more pronounced. This study presents a rapid and reproducible in situ method to estimate biofilm-associated enzymatic activity using hydrogen peroxide (H₂O₂) degradation. Colonized bioelements were collected from a moving bed biofilter in a commercial trout RAS and transferred to a standardized setup where H<sub>2</sub>O<sub>2</sub> was added and measured over a 20-min period. Strong correlations were found between the number of bioelements and the degradation rate of H<sub>2</sub>O<sub>2</sub> across three independent trials. The method was then tested on bioelements exposed to severe disinfection, showing a 92% reduction. Autoclaved bioelements (controls) showed negligible H₂O₂ removal capacity, corresponding to ≥ 99.5% inhibition compared to active bioelements. Although the method does not yield absolute values and is dependent on test conditions, it proved highly reproducible with clear signals and minimal noise. This quick approach can provide new knowledge and support better understanding and management of microbial processes in RAS.</p>

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A rapid method to estimate microbial activity in biofilter biofilm using hydrogen peroxide degradation

  • 期刊论文

  • 发表日期：

    2025年11月7日

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  • Lars-Flemming Pedersen

摘要

Microbes pose a central role in successful management of recirculating aquaculture systems (RAS). As a result, the need to monitor and quantify the planktonic and surface-oriented microorganisms is becoming more pronounced. This study presents a rapid and reproducible in situ method to estimate biofilm-associated enzymatic activity using hydrogen peroxide (H₂O₂) degradation. Colonized bioelements were collected from a moving bed biofilter in a commercial trout RAS and transferred to a standardized setup where H2O2 was added and measured over a 20-min period. Strong correlations were found between the number of bioelements and the degradation rate of H2O2 across three independent trials. The method was then tested on bioelements exposed to severe disinfection, showing a 92% reduction. Autoclaved bioelements (controls) showed negligible H₂O₂ removal capacity, corresponding to ≥ 99.5% inhibition compared to active bioelements. Although the method does not yield absolute values and is dependent on test conditions, it proved highly reproducible with clear signals and minimal noise. This quick approach can provide new knowledge and support better understanding and management of microbial processes in RAS.