Scholarlink

<p><i>Vibrio misgurnus</i>, an emerging aquaculture pathogen, causes high-mortality outbreaks in farmed <i>Misgurnus anguillicaudatus</i> with significant economic impacts. The <i>Vibrio misgurnus</i> GVC exhibited multiple antibiotic resistance. However, there are currently no effective antibiotic alternatives for GVC. Magnolol, a bioactive bisphenol compound derived from <i>Magnolia officinalis</i>, has demonstrated broad-spectrum antibacterial properties and proven efficacy against various bacterial infections. This study investigated magnolol’s inhibitory effects on GVC through phenotypic assays and transcriptomic analysis. The MIC and MBC of magnolol against GVC were 64 and 128 μg/mL. Transcriptomic profiling enriched 638 upregulated and 660 downregulated genes. KEGG enrichment showed that the expression levels of DEGs in the GVC ribosome pathway were all upregulated, accompanied by complex expression patterns in critical metabolic and pathogenic pathways, including glycolysis/gluconeogenesis, signal transduction, virulence, motility, and ABC transporters. Magnolol treatment at varying concentrations (1/4 MIC, 1/2 MIC, and MIC) inhibited GVC growth and biofilm formation, compromising membrane integrity and leading to DNA leakage. Protective experiments demonstrated that magnolol treatment at 20 and 40 mg/kg reduced mortality in GVC-infected <i>M. anguillicaudatus</i> by 20.0% and 53.3%, respectively. Additionally, magnolol disrupted the synthesis of most soluble proteins larger than 35 kDa in GVC. Molecular docking further revealed a high affinity between magnolol and ribosomal proteins (RplB, RplD, RplE, RplP, RpsC, and RpsD). Our findings provide novel insights into magnolol’s inhibition against GVC and highlight its potential as an alternative agent for controlling <i>Vibrio misgurnus</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Phenotypic and transcriptomic insights into the antibacterial effects of magnolol against the novel pathogen Vibrio misgurnus

  • 期刊论文

  • 发表日期：

    2025年8月2日

查看全文
  • Keyu Zhou,
  • Qibin Jiang,
  • Bowen Huang,
  • Kun Peng,
  • Mingqi Ai,
  • Le Xu,
  • Songmao Wu,
  • Ping Ouyang,
  • Xiaoli Huang,
  • Defang Chen,
  • Yi Geng

摘要

Vibrio misgurnus, an emerging aquaculture pathogen, causes high-mortality outbreaks in farmed Misgurnus anguillicaudatus with significant economic impacts. The Vibrio misgurnus GVC exhibited multiple antibiotic resistance. However, there are currently no effective antibiotic alternatives for GVC. Magnolol, a bioactive bisphenol compound derived from Magnolia officinalis, has demonstrated broad-spectrum antibacterial properties and proven efficacy against various bacterial infections. This study investigated magnolol’s inhibitory effects on GVC through phenotypic assays and transcriptomic analysis. The MIC and MBC of magnolol against GVC were 64 and 128 μg/mL. Transcriptomic profiling enriched 638 upregulated and 660 downregulated genes. KEGG enrichment showed that the expression levels of DEGs in the GVC ribosome pathway were all upregulated, accompanied by complex expression patterns in critical metabolic and pathogenic pathways, including glycolysis/gluconeogenesis, signal transduction, virulence, motility, and ABC transporters. Magnolol treatment at varying concentrations (1/4 MIC, 1/2 MIC, and MIC) inhibited GVC growth and biofilm formation, compromising membrane integrity and leading to DNA leakage. Protective experiments demonstrated that magnolol treatment at 20 and 40 mg/kg reduced mortality in GVC-infected M. anguillicaudatus by 20.0% and 53.3%, respectively. Additionally, magnolol disrupted the synthesis of most soluble proteins larger than 35 kDa in GVC. Molecular docking further revealed a high affinity between magnolol and ribosomal proteins (RplB, RplD, RplE, RplP, RpsC, and RpsD). Our findings provide novel insights into magnolol’s inhibition against GVC and highlight its potential as an alternative agent for controlling Vibrio misgurnus.