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<p>Fat greenling (<i>Hexagrammos otakii</i>) is an important marine aquaculture species in northern China. In recent years, the increase in global surface seawater temperatures has led to frequent extreme high-temperature events in summer. This rise is affecting culture sites in northern China with a predicted increase of 0.5&#xa0;°C to 3.0&#xa0;°C over the next fifty years. In order to better understand the tolerance of fat greenling to different temperatures, the impacts of temperature stress on growth, histology, oxidative stress levels, and transcriptome in liver tissue were studied. Growth performance and feed conversion ratio were significantly lower and higher, respectively, in the experimental groups (5&#xa0;°C, 10&#xa0;°C, 20&#xa0;°C, 25&#xa0;°C) compared to the control group (15&#xa0;°C). Furthermore, the histology results showed that liver tissue from the 25&#xa0;°C group exhibited pathological features, including nuclear dissolution, cytoplasmic loss, and indistinct cell boundaries. Additionally, the activities of antioxidant enzymes were increased significantly to cope with temperature stress. Transcriptome analysis revealed significant differences in fat greenling liver gene expression between temperature treatments (5&#xa0;°C, 15&#xa0;°C, and 25&#xa0;°C). Differential expression was found at genes associated with heat production and metabolism, reflecting the body's adaptation to different temperatures. The results of temperature stress on growth, histology, oxidative stress levels, and transcriptome in liver collectively suggest that the optimal growth temperature for fat greenling ranged from 15&#xa0;°C to 20&#xa0;°C. This study provides specific insights into how <i>H. otakii</i> adjusts its growth performance, antioxidant defense system, and liver metabolism in response to temperature fluctuations, offering valuable references for temperature management strategies in aquaculture.</p>

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Effects of temperature stress on liver histology, oxidative stress, and transcriptome in fat greenling (Hexagrammos otakii)

  • 期刊论文

  • 发表日期：

    2025年6月30日

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  • Zheng Zhang,
  • Yanchao Wei,
  • Hui Liu,
  • Shuai Wang,
  • Yanyan Shi,
  • Dandan Cui,
  • Yan Chen,
  • Xuejie Li,
  • Wei Wang

摘要

Fat greenling (Hexagrammos otakii) is an important marine aquaculture species in northern China. In recent years, the increase in global surface seawater temperatures has led to frequent extreme high-temperature events in summer. This rise is affecting culture sites in northern China with a predicted increase of 0.5 °C to 3.0 °C over the next fifty years. In order to better understand the tolerance of fat greenling to different temperatures, the impacts of temperature stress on growth, histology, oxidative stress levels, and transcriptome in liver tissue were studied. Growth performance and feed conversion ratio were significantly lower and higher, respectively, in the experimental groups (5 °C, 10 °C, 20 °C, 25 °C) compared to the control group (15 °C). Furthermore, the histology results showed that liver tissue from the 25 °C group exhibited pathological features, including nuclear dissolution, cytoplasmic loss, and indistinct cell boundaries. Additionally, the activities of antioxidant enzymes were increased significantly to cope with temperature stress. Transcriptome analysis revealed significant differences in fat greenling liver gene expression between temperature treatments (5 °C, 15 °C, and 25 °C). Differential expression was found at genes associated with heat production and metabolism, reflecting the body's adaptation to different temperatures. The results of temperature stress on growth, histology, oxidative stress levels, and transcriptome in liver collectively suggest that the optimal growth temperature for fat greenling ranged from 15 °C to 20 °C. This study provides specific insights into how H. otakii adjusts its growth performance, antioxidant defense system, and liver metabolism in response to temperature fluctuations, offering valuable references for temperature management strategies in aquaculture.