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<p>Hypoxia upregulated 1 (Hyou1), a member of the heat shock protein 70 (HSP70) family, plays a crucial role in promoting cell survival and cytoprotection under hypoxic stress. However, the phylogenetic relationship and function of Hyou1 in molluscs remain poorly characterized. The razor clam <i>Sinonovacula constricta</i>, frequently subjected to hypoxic stress due to its deep-burrowing lifestyle and high-density pond aquaculture conditions, serves as an ideal model for studying hypoxia adaptation mechanisms. In this study, we investigated the phylogenetic relationships of Hyou1 in bivalves, its tissue distribution patterns, and the effect of <i>Hyou1</i> gene interference on hepatopancreas cell apoptosis in <i>S. constricta</i>. The Hyou1 gene in <i>S. constricta</i> (<i>Sc-Hyou1</i>) displayed high amino acid sequence identity with species of the Venerida order. Among the studied molluscs, exons 3–12, formed the N-terminal nucleotide-binding domain (NBD), were conserved in size and contained highly conserved sequence. Furthermore, 18 positively selected sites were identified in the Hyou1 coding sequences across 10 molluscan species, with 14 located in the NBD and 4 in the substrate-binding domain. Additionally, <i>Sc-Hyou1</i> was predominantly expressed in the hepatopancreas, and its interference significantly increased hepatopancreatic cell apoptosis under hypoxic stress. Taken together, our findings suggest that Hyou1 undergoes strong positive selection in <i>S. constricta</i> and may mediate anti-apoptotic response to hypoxia. These results provide new insights into the evolutionary significance and molecular mechanisms of <i>Hyou1</i> in bivalves.</p>

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Adaptive evolution, expression, and functional analysis of Hyou1 gene in response to hypoxic stress in razor clam (Sinonovacula constricta)

  • 期刊论文

  • 发表日期：

    2025年6月7日

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  • Ran Sun,
  • Zhihua Lin,
  • Hanhan Yao,
  • Liyuan Lv,
  • Yinghui Dong

摘要

Hypoxia upregulated 1 (Hyou1), a member of the heat shock protein 70 (HSP70) family, plays a crucial role in promoting cell survival and cytoprotection under hypoxic stress. However, the phylogenetic relationship and function of Hyou1 in molluscs remain poorly characterized. The razor clam Sinonovacula constricta, frequently subjected to hypoxic stress due to its deep-burrowing lifestyle and high-density pond aquaculture conditions, serves as an ideal model for studying hypoxia adaptation mechanisms. In this study, we investigated the phylogenetic relationships of Hyou1 in bivalves, its tissue distribution patterns, and the effect of Hyou1 gene interference on hepatopancreas cell apoptosis in S. constricta. The Hyou1 gene in S. constricta (Sc-Hyou1) displayed high amino acid sequence identity with species of the Venerida order. Among the studied molluscs, exons 3–12, formed the N-terminal nucleotide-binding domain (NBD), were conserved in size and contained highly conserved sequence. Furthermore, 18 positively selected sites were identified in the Hyou1 coding sequences across 10 molluscan species, with 14 located in the NBD and 4 in the substrate-binding domain. Additionally, Sc-Hyou1 was predominantly expressed in the hepatopancreas, and its interference significantly increased hepatopancreatic cell apoptosis under hypoxic stress. Taken together, our findings suggest that Hyou1 undergoes strong positive selection in S. constricta and may mediate anti-apoptotic response to hypoxia. These results provide new insights into the evolutionary significance and molecular mechanisms of Hyou1 in bivalves.