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<p>Myo-inositol (MI) plays a vital role in the growth and development of crustaceans. Although most researchers have focused on the critical role of MI in fish, its application in crustaceans is lacking. A 6-week trial was conducted to comparatively evaluate the effects of 6 levels of dietary MI (0, 500, 1000, 2000, 3000, and 4000&#xa0;mg/kg diet) in juvenile freshwater crayfish <i>Procambarus clarkii</i> (6.39 ± 0.05) g. This study aimed to investigate the effects of dietary MI on antioxidant capacity, non-specific immunity, and possible molecular mechanisms in freshwater crayfish. Research shows dietary MI could improve hepatopancreatic antioxidant enzyme activity and non-specific immunity (<i>P</i> &lt; 0.05). MI can be converted into phosphatidylinositol 3-kinase (PI3K) under the action of phospholipase, which activates the PI3K/AKT signaling pathway. The PI3K/AKT signaling pathway can promote the transcription level of Nrf2-Keap1, thereby promoting the expression of antioxidant-related genes (<i>sod</i>, <i>cat</i>, and <i>gpx</i>) to improve the antioxidant mechanism (<i>P</i> &lt; 0.05). In addition, dietary MI could prevent the occurrence of endoplasmic reticulum stress (ERS, <i>bip</i>, <i>xbp1</i>, and <i>ire1</i>) and inhibit the expression of inflammatory factors (<i>jnk</i>, <i>il-6</i>, <i>tnf-</i>α, and <i>nf-κb</i>) and apoptosis-related genes (<i>bax</i>, <i>cytc</i>, and <i>casp3</i>) (<i>P</i> &lt; 0.05). Dietary MI improves antioxidant capacity by regulating increased Nrf2 translocation and NF-κB translocation. In conclusion, dietary MI can enhance the antioxidant defense system of freshwater crayfish by activating the PI3K/AKT pathway and coordinating the transcription levels of Nrf2 and NF-κB.</p>

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The effects of Myo-inositol on antioxidant capacity and immunity in freshwater crayfish

  • 期刊论文

  • 发表日期：

    2025年4月3日

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  • Changchang Pu,
  • Yuanyi Liu,
  • Yinfeng Cheng,
  • Bingke Wang,
  • Aimin Wang,
  • Chunnuan Zhang

摘要

Myo-inositol (MI) plays a vital role in the growth and development of crustaceans. Although most researchers have focused on the critical role of MI in fish, its application in crustaceans is lacking. A 6-week trial was conducted to comparatively evaluate the effects of 6 levels of dietary MI (0, 500, 1000, 2000, 3000, and 4000 mg/kg diet) in juvenile freshwater crayfish Procambarus clarkii (6.39 ± 0.05) g. This study aimed to investigate the effects of dietary MI on antioxidant capacity, non-specific immunity, and possible molecular mechanisms in freshwater crayfish. Research shows dietary MI could improve hepatopancreatic antioxidant enzyme activity and non-specific immunity (P < 0.05). MI can be converted into phosphatidylinositol 3-kinase (PI3K) under the action of phospholipase, which activates the PI3K/AKT signaling pathway. The PI3K/AKT signaling pathway can promote the transcription level of Nrf2-Keap1, thereby promoting the expression of antioxidant-related genes (sod, cat, and gpx) to improve the antioxidant mechanism (P < 0.05). In addition, dietary MI could prevent the occurrence of endoplasmic reticulum stress (ERS, bip, xbp1, and ire1) and inhibit the expression of inflammatory factors (jnk, il-6, tnf-α, and nf-κb) and apoptosis-related genes (bax, cytc, and casp3) (P < 0.05). Dietary MI improves antioxidant capacity by regulating increased Nrf2 translocation and NF-κB translocation. In conclusion, dietary MI can enhance the antioxidant defense system of freshwater crayfish by activating the PI3K/AKT pathway and coordinating the transcription levels of Nrf2 and NF-κB.