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<p>The ethylmalonic encephalopathy protein 1 (ETHE1/SDO) plays a pivotal role in sulfide metabolism and is involved in regulating the organism's tolerance to hypoxic environments. Nevertheless, the function of ETHE1 in <i>Litopenaeus vannamei</i>, particularly under hypoxic conditions, remains poorly understood. In this study, we amplified the <i>ETHE1</i> sequence of <i>L. vannamei</i> using PCR, which encompasses an 864&#xa0;bp open reading frame (ORF) encoding a 287 amino acid polypeptide with a highly conserved Lactamase_B domain. RT-qPCR results demonstrated ubiquitous expression of <i>LvETHE1</i> across all tissues, with higher levels observed in the gill, muscle and hepatopancreas, and the lowest levels found in the abdominal ganglia. Hypoxia stress significantly upregulated the expression of <i>LvETHE1</i> in the gill, hepatopancreas, muscle, and intestine (<i>P</i> &lt; 0.05). <i>LvETHE1</i> RNA interference under hypoxia stress markedly increased the expression of antioxidant genes (<i>Nrf2, HO-1, Mn-SOD, CAT, GPX, and GST</i>) as well as apoptosis-related genes (<i>Caspase-3, Cyt-C, P53, Bax</i>) in both gill and hepatopancreas (<i>P</i> &lt; 0.05). The content of MAD and the activities of CAT, GPX, and SOD were variably enhanced in the gill and hepatopancreas. The injection of ds<i>LvETHE1</i> RNAi under hypoxia stress resulted in augmented apoptosis signals and more pronounced structural damage in the gill and hepatopancreas, as evidenced by TUNEL staining and histological observation. Moreover, it significantly increased shrimp mortality under hypoxia stress. These findings collectively indicated that <i>LvETHE1</i> is implicated in regulating antioxidant capacity, oxidative stress, and apoptosis processes within <i>L. vannamei.</i> The findings of this study hold significant importance for promoting prawn health and sustainable culture.</p>

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The ethylmalonic encephalopathy protein 1 (ETHE1) is involved in the regulation of antioxidation and apoptosis in Litopenaeus vannamei under hypoxia stress

  • 期刊论文

  • 发表日期：

    2025年3月26日

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  • Guangbo Wu,
  • Xiaoxun Zhou,
  • Shuo Fu,
  • Yongkang Hou,
  • Chaoan Guo,
  • Jianyong Liu

摘要

The ethylmalonic encephalopathy protein 1 (ETHE1/SDO) plays a pivotal role in sulfide metabolism and is involved in regulating the organism's tolerance to hypoxic environments. Nevertheless, the function of ETHE1 in Litopenaeus vannamei, particularly under hypoxic conditions, remains poorly understood. In this study, we amplified the ETHE1 sequence of L. vannamei using PCR, which encompasses an 864 bp open reading frame (ORF) encoding a 287 amino acid polypeptide with a highly conserved Lactamase_B domain. RT-qPCR results demonstrated ubiquitous expression of LvETHE1 across all tissues, with higher levels observed in the gill, muscle and hepatopancreas, and the lowest levels found in the abdominal ganglia. Hypoxia stress significantly upregulated the expression of LvETHE1 in the gill, hepatopancreas, muscle, and intestine (P < 0.05). LvETHE1 RNA interference under hypoxia stress markedly increased the expression of antioxidant genes (Nrf2, HO-1, Mn-SOD, CAT, GPX, and GST) as well as apoptosis-related genes (Caspase-3, Cyt-C, P53, Bax) in both gill and hepatopancreas (P < 0.05). The content of MAD and the activities of CAT, GPX, and SOD were variably enhanced in the gill and hepatopancreas. The injection of dsLvETHE1 RNAi under hypoxia stress resulted in augmented apoptosis signals and more pronounced structural damage in the gill and hepatopancreas, as evidenced by TUNEL staining and histological observation. Moreover, it significantly increased shrimp mortality under hypoxia stress. These findings collectively indicated that LvETHE1 is implicated in regulating antioxidant capacity, oxidative stress, and apoptosis processes within L. vannamei. The findings of this study hold significant importance for promoting prawn health and sustainable culture.