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<p>Phage therapy represents a promising alternative in aquaculture to control bacterial infections, helping to manage antibiotic resistance and promote healthier aquatic environments. In the present work, the phage vB_Vp_PvVp04 genome and its host range were described. Four strategies for the incorporation of this phage in shrimp <i>Penaeus vannamei</i> feed were explored: directly included as liquid (IL), encapsulation (EN), freeze-drying (FD), and encapsulation + freeze-drying (EN + FD). The results showed that vB_Vp_PvVp04 infects <i>Vibrio parahaemolyticus</i> CIBGEN003 and inhibited growth by 87.6% after 12&#xa0;h. Its host range includes other <i>Vibrio</i> species. Its genome, 39,824&#xa0;bp in length and lacking tRNA or antibiotic resistance genes, contains 67 ORFs. Phylogenetic analysis grouped vB_Vp_PvVp04 with other <i>Myoviridae</i> phages. During storage stability testing, diets containing vB_Vp_PvVp04 maintained stable viability over 90&#xa0;days, except in IL formulations, which showed a significant reduction. The FD and EN + FD phages showed greater presence in the shrimp stomach, reaching 10<sup>11</sup> PFU/g, which would demonstrate that these methods for phage inclusion in feed can be a viable strategy for controlling opportunistic pathogens in white shrimp farming.</p>

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A novel vibriophage vB_Vp_PvVp04 against pathogenic Vibrio parahaemolyticus and its formulation for inclusion in shrimp feed

  • 期刊论文

  • 发表日期：

    2025年1月2日

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  • Alberto Peña-Rodríguez,
  • Irais Ramírez-Sánchez,
  • Sebastián Leptihn,
  • Eduardo Quiroz-Guzmán

摘要

Phage therapy represents a promising alternative in aquaculture to control bacterial infections, helping to manage antibiotic resistance and promote healthier aquatic environments. In the present work, the phage vB_Vp_PvVp04 genome and its host range were described. Four strategies for the incorporation of this phage in shrimp Penaeus vannamei feed were explored: directly included as liquid (IL), encapsulation (EN), freeze-drying (FD), and encapsulation + freeze-drying (EN + FD). The results showed that vB_Vp_PvVp04 infects Vibrio parahaemolyticus CIBGEN003 and inhibited growth by 87.6% after 12 h. Its host range includes other Vibrio species. Its genome, 39,824 bp in length and lacking tRNA or antibiotic resistance genes, contains 67 ORFs. Phylogenetic analysis grouped vB_Vp_PvVp04 with other Myoviridae phages. During storage stability testing, diets containing vB_Vp_PvVp04 maintained stable viability over 90 days, except in IL formulations, which showed a significant reduction. The FD and EN + FD phages showed greater presence in the shrimp stomach, reaching 1011 PFU/g, which would demonstrate that these methods for phage inclusion in feed can be a viable strategy for controlling opportunistic pathogens in white shrimp farming.