<p>Acoustic technology emerges as a new tool for ethological studies as shrimp mandibles emit click sounds during feeding activities. Passive acoustic monitoring (PAM) was applied for the first time to evaluate the feeding behavior of <i>Litopenaeus vannamei</i> subjected to different feeding frequencies. Shrimp groups (mean weight of 3.31&#xa0;g) were distributed in 12 tanks, corresponding to three treatment replicates for feeding frequencies of one, two, four, and eight times a day. In these treatments, the same amount of pelleted feed was offered either in a single feeding or divided into two, four, or eight daily portions. Feeding activity was monitored during eight hours (8 am to 4 pm) using hydrophones connected to a digital recorder. At the end of the recordings, the uneaten feed was collected to estimate the feed consumption. Audio recordings were analyzed using Raven^® Pro 1.5 software. The feeding behavior of <i>L. vannamei</i> was affected by the feeding frequencies from once to eight times a day, with higher values for the number of clicks emitted and feed consumption in shrimp groups fed more frequently. In all feeding frequencies, there was an initial peak in the number of clicks associated with the first feeding of the day. However, new peaks in acoustic activity occurred only after subsequent feedings in the groups subjected to feeding freque ncies of two, four, and eight times a day. Results indicated benefits in employing multiple feeding frequencies of up to eight times a day to increase feeding activity and feed consumption in <i>L. vannamei</i>.</p>