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<p>Sphingomyelin synthase (SMS) is a key enzyme in sphingolipid metabolism that catalyzes the synthesis of sphingomyelin (SM). The potential roles of SMS involved in the egg development and female fecundity on the tremendous pest mite <i>Panonychus citri</i>, however, remain unknown. In this study, we determined the function of sphingomyelin synthase gene in <i>P. citri</i> across different developmental stages. We silenced the <i>PcSMS</i> in adult females of <i>P. citri</i> via feeding on detached citrus leaves that absorbed dsRNAs. The survival rates and lifespan, along with the expressions of ROS and apoptosis genes were significantly changed. Additionally, fecundity and oviposition periods of <i>P. citri</i> were shortened. The expression levels of vitellogenin and its receptor genes were downregulated. Knockdown of <i>PcSMS</i> also affected egg hatching, resulting in developmental delays and egg lethal phenotypes. Our findings shed light on the metabolism underlying the biological functions of egg development and adult females fecundity of <i>P. citri</i> and render lipid metabolism the attractive target for biopesticide to control citrus spider mite.</p>

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RNAi-mediated knockdown of sphingomyelin synthase gene affects egg development and female fecundity in Panonychus citri (McGregor) (Acari: Tetranychidae)

  • 期刊论文

  • 发表日期：

    2026年2月2日

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  • Ying Xie,
  • Lulu Chen,
  • Jiaying Wang,
  • Aoqi Feng,
  • Haifeng Wang,
  • Zhiwen Zou,
  • Bin Xia,
  • Tianrong Xin

摘要

Sphingomyelin synthase (SMS) is a key enzyme in sphingolipid metabolism that catalyzes the synthesis of sphingomyelin (SM). The potential roles of SMS involved in the egg development and female fecundity on the tremendous pest mite Panonychus citri, however, remain unknown. In this study, we determined the function of sphingomyelin synthase gene in P. citri across different developmental stages. We silenced the PcSMS in adult females of P. citri via feeding on detached citrus leaves that absorbed dsRNAs. The survival rates and lifespan, along with the expressions of ROS and apoptosis genes were significantly changed. Additionally, fecundity and oviposition periods of P. citri were shortened. The expression levels of vitellogenin and its receptor genes were downregulated. Knockdown of PcSMS also affected egg hatching, resulting in developmental delays and egg lethal phenotypes. Our findings shed light on the metabolism underlying the biological functions of egg development and adult females fecundity of P. citri and render lipid metabolism the attractive target for biopesticide to control citrus spider mite.